Related Experiment Video

Updated: Aug 13, 2025

Injections of Lipopolysaccharide into Mice to Mimic Entrance of Microbial-derived Products After Intestinal Barrier Breach
08:24

Injections of Lipopolysaccharide into Mice to Mimic Entrance of Microbial-derived Products After Intestinal Barrier Breach

Published on: May 2, 2018

19.7K

Crosstalk between imbalanced gut microbiota caused by antibiotic exposure and rotavirus replication in the intestine

Yuhui Li1, Yifan Wu2, Jie Wu2

  • 1School of Biology, Food and Environment, Hefei University, Hefei 230032, Anhui, China.

Heliyon
|January 23, 2023
PubMed

Insights

Asymptomatic adults can spread rotavirus (RV). Antibiotic exposure disrupts gut bacteria, increasing RV replication risk and potential health issues. Further research is needed.

Area of Science:

  • Virology
  • Microbiology
  • Gastroenterology

Background:

  • Rotavirus (RV) is a major cause of infantile diarrhea, primarily transmitted via the fecal-oral route.
  • Asymptomatic adult carriers are increasingly recognized as a significant source of RV transmission.
  • Understanding RV replication mechanisms in adult intestines is crucial for public health.

Purpose of the Study:

  • To investigate the link between animal-derived food intake and antibiotic levels in adults.
  • To compare gut microbiota structures in RV carriers versus healthy individuals.
  • To determine the impact of lipopolysaccharide (LPS) on RV replication.

Main Methods:

  • Recruitment of healthy volunteers and asymptomatic RV carriers.
  • Analysis of urine antibiotic levels and gut microbiota composition.
  • In vivo and in vitro experiments assessing LPS effects on RV replication.

Main Results:

  • 10% of participants were asymptomatic RV carriers.
  • High animal-derived food intake correlated with elevated urine antibiotic levels.
  • RV carriers exhibited disrupted gut microbiota with antibiotic-resistant gram-negative bacteria and high LPS levels.
  • Penicillin-induced gut dysbiosis and LPS exposure promoted RV replication.

Conclusions:

  • Imbalanced gut microbiota due to antibiotic exposure is a key factor in RV replication.
  • This dysbiosis increases the risk of RV-related health complications.
  • Findings highlight the importance of gut health in preventing viral infections.
Abstract

Keywords:
AntibioticsGut microbiotaLPSResistant genesRotavirus

More Related Videos

Quantitative Polymerase Chain Reaction-based Analyses of Murine Intestinal Microbiota After Oral Antibiotic Treatment08:33

Quantitative Polymerase Chain Reaction-based Analyses of Murine Intestinal Microbiota After Oral Antibiotic Treatment

Published on: November 17, 2018

13.1K
Induction of Intestinal Inflammation by Adoptive Transfer of CBir1 TCR Transgenic CD4+ T Cells to Immunodeficient Mice07:34

Induction of Intestinal Inflammation by Adoptive Transfer of CBir1 TCR Transgenic CD4+ T Cells to Immunodeficient Mice

Published on: December 16, 2021

2.6K

Related Concept Videos

Drugs Affecting GI Tract Motility: Antimicrobials as Antidiarrheal Agents01:18

Drugs Affecting GI Tract Motility: Antimicrobials as Antidiarrheal Agents

Acute diarrhea, a common gastrointestinal disturbance, is characterized by the rapid evacuation of fluid stools, leading to an excessive weight in fluid. This condition typically arises from disorders affecting intestinal water and electrolyte transport. It can be triggered by an increased osmotic load within the intestine, excessive secretion of electrolytes and water, mucosal exudation of protein and fluid, or altered intestinal motility. The primary risks of acute diarrhea are dehydration...
190
Anatomy of the Intestines01:23

Anatomy of the Intestines

Although digestion of proteins, carbohydrates, and lipids may begin in the stomach, it is completed in the intestine. The absorption of nutrients, water, and electrolytes from food and drink also occurs in the intestine. The intestines can be divided into two structurally distinct organs—the small and large intestines.
Small Intestines
The small intestine is an ~7 meter-long tube with an inner diameter of just 2.5 cm. Since most nutrients are absorbed here, the inner lining of the...
72.9K
Irritable Bowel Syndrome I: Introduction01:17

Irritable Bowel Syndrome I: Introduction

Irritable Bowel Syndrome (IBS) is characterized by functional disturbances in the gastrointestinal system, presenting a cluster of symptoms without evident structural or biochemical abnormalities. It primarily affects the large intestine and may cause abdominal pain, bloating, excessive gas, diarrhea, constipation, or both.
IBS is a chronic condition that can persist over a long period or recur frequently.
The pathogenesis of IBS involves a complex interplay of the following factors:
Altered...
347
Bacterial Flora of the Large Intestine01:29

Bacterial Flora of the Large Intestine

The gut microbiome is formed by a vast and diverse community of bacteria that colonizes our large intestine. These bacteria start residing in the gut from birth and continue diversifying throughout life, influenced by factors such as diet, lifestyle, and stress. The gut bacterial community also includes bacteria from food and those that enter the colon through the anus.
The normal gut flora of the colon plays a critical role in generating essential vitamins such as vitamins K, B5, and B7.
558
What is Monogastric Digestion?01:50

What is Monogastric Digestion?

The human body contains a monogastric digestive system. In a monogastric digestive system, the stomach only contains one chamber in which it digests food. Several other animal species also have monogastric digestive systems, including pigs, horses, dogs, and birds. This chapter, however, focuses on the human digestive system.
71.9K