Related Experiment Video
Updated: Aug 13, 2025
A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses
Published on: January 25, 2018
Analysis of genotype × environment interactions for agronomic traits of soybean (Glycine max [L.] Merr.) using
Reena Rani1, Ghulam Raza1, Hamza Ashfaq1
1DNA Markers and Applied Genomics Lab, Agricultural Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Constituent College Pakistan Institute of Engineering and Applied Sciences (PIEAS), Faisalabad, Pakistan.
Abstract:
The soybean yield is a complex quantitative trait that is significantly influenced by environmental factors. G × E interaction (GEI), which derives the performance of soybean genotypes differentially in various environmental conditions, is one of the main obstacles to increasing the net production. The primary goal of this study is to identify the outperforming genotypes in different latitudes, which can then be used in future breeding programs. A total of 96 soybean genotypes were examined in two different ecological regions: Faisalabad and Tando Jam in Pakistan. The evaluation of genotypes in different environmental conditions showed a substantial amount of genetic diversity for grain yield. We identified 13 environment-specific genotypes showing their maximum grain yield in each environment. Genotype G69 was found to be an ideal genotype with higher grain yield than other genotypes tested in this study and is broadly adapted for environments E1 and E2 and also included in top-yielding genotypes in E3, E4, and E5. G92 is another genotype that is broadly adapted in E1, E3, and E4. In the case of environments, E3 is suggested to be a more ideal environment as it is plotted near the concentric circle and is very informative for the selection of genotypes with high yield. Despite the presence of GEI, advances in DNA technology provided very useful tools to investigate the insight of advanced genotypes. Association mapping is a useful method for swiftly and efficiently investigating the genetic basis of significant plant traits. A total of 26 marker-trait associations were found for six agronomic traits in five environments, with the highest significance (p-value = 2.48 × 10-08) for plant height and the lowest significance (1.03 × 10-03) for hundred-grain weight. Soybean genotypes identified in the present study could be a valuable source for future breeding programs as they are adaptable to a wide range of environments. Genetic selection of genotypes with the best yields can be used for gross grain production in a wide range of climatic conditions, and it would give an essential reference in terms of soybean variety selection.
Related Concept Videos
02:27Background and Environment Affect Phenotype
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
01:09Epistasis Analysis
01:20Gene-Environment Interactions
02:16Light Acquisition
01:18Dihybrid Crosses
01:25Multiple Regression
Farmers can use multiple regression to determine the crop yield based on more than one factor, such as water availability, fertilizer, soil properties, etc. Here, the crop yield is the response or dependent variable as it depends on the other independent variables. The analysis requires the construction of a scatter plot...