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Updated: Aug 13, 2025

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

Published on: May 16, 2022

Bioimaging with Upconversion Nanoparticles.

Evan M Mettenbrink¹, Wen Yang¹, Stefan Wilhelm^1,2,3

¹Stephenson School of Biomedical Engineering, University of Oklahoma, Norman, Oklahoma, 73019, USA.

Advanced Photonics Research

|January 23, 2023

Summary

This summary is machine-generated.

Upconversion nanoparticles (UCNPs) offer unique optical properties for advanced bioimaging. This review covers UCNP applications from cellular to whole-animal levels, discussing opportunities and future potential in clinical translation.

Keywords:

Upconversion bioimaging in vivo imaging luminescence microscopy multimodal nanoparticles super-resolution

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Area of Science:

Nanotechnology
Biomedical Imaging
Materials Science

Background:

Bioimaging visualizes biological processes using various modalities and contrast agents.
Upconversion nanoparticles (UCNPs) possess unique optical properties and design flexibility, positioning them as advanced contrast agents.

Purpose of the Study:

To review the opportunities, challenges, and limitations of UCNPs as bioimaging probes.
To highlight UCNP applications across different spatial scales (nanoparticle, cellular, tissue, whole animal).
To summarize advancements in UCNP-based super-resolution and multimodal imaging.

Main Methods:

Literature review of UCNP applications in bioimaging.
Discussion of UCNP properties and functionalization strategies.
Analysis of current and emerging UCNP-based imaging techniques.

Main Results:

UCNPs demonstrate significant potential for various bioimaging scales due to their distinct optical characteristics.
Recent advancements include UCNP integration into super-resolution and multimodal imaging platforms.
Challenges include optimizing UCNP synthesis, surface modification, and in vivo performance.

Conclusions:

UCNPs represent a promising technology for advancing bioimaging research.
Further development is crucial for successful clinical translation of UCNP-based diagnostics and therapeutics.
This review provides a comprehensive resource for researchers in UCNP bioimaging.