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Updated: Aug 13, 2025

Primer-Free Aptamer Selection Using A Random DNA Library
Published on: July 26, 2010
Selection methods for proximity-dependent enrichment of ligands from DNA-encoded libraries using enzymatic fusion
Bo Cai1, Amol B Mhetre1, Casey J Krusemark1
1Department of Medicinal Chemistry and Molecular Pharmacology, Purdue Center for Cancer Research, Purdue University West Lafayette IN 47907 USA cjk@purdue.edu.
Abstract:
Herein, we report a selection approach to enrich ligands from DNA-encoded libraries (DELs) based on proximity to an enzymatic tag on the target protein. This method involves uncaging or installation of a biotin purification tag on the DNA construct either through photodeprotection of a protected biotin group using a light emitting protein tag (nanoluciferase) or by acylation using an engineered biotin ligase (UltraID). This selection does not require purification of the target protein and results in improved recovery and enrichment of DNA-linked ligands. This approach should serve as a general and convenient tool for molecular discovery with DELs.
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