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Related Experiment Video

Updated: Aug 13, 2025

Dissolved Solute Sampling Across an Oxic-Anoxic Soil-Water Interface Using Microdialysis Profilers

Dissolved Solute Sampling Across an Oxic-Anoxic Soil-Water Interface Using Microdialysis Profilers

Published on: March 24, 2023

A deep continental aquifer downhole sampler for microbiological studies.

Magali Ranchou-Peyruse^1,2,3, Marion Guignard¹, Perla G Haddad²

¹E2S-UPPA, CNRS, IPREM, Universite de Pau & Pays Adour, Pau, France.

Frontiers in Microbiology

|January 23, 2023

Summary

Microbiological studies of deep aquifers require pure water samples. A specialized downhole sampler effectively prevents surface contamination, ensuring accurate analysis of deep biosphere microbial communities.

Keywords:

ATEX UGS deep aquifer downhole sampler geological storage microorganisms

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Last Updated: Aug 13, 2025

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Published on: March 24, 2023

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VacuSIP, an Improved InEx Method for In Situ Measurement of Particulate and Dissolved Compounds Processed by Active Suspension Feeders

Published on: August 3, 2016

Area of Science:

Environmental microbiology
Geomicrobiology
Deep biosphere research

Background:

Effective microbiological studies of deep aquifers necessitate pristine water samples, free from surface contaminants and wellbore-associated biofilms.
Existing methods relying on wellhead sampling after rinsing often fail to eliminate microbial contamination, compromising study integrity.

Purpose of the Study:

To present and validate a protocol for adapting, sterilizing, and deploying a commercial downhole sampler for deep aquifer microbiological studies.
To demonstrate the necessity of downhole sampling for obtaining uncontaminated formation water and preserving *in situ* conditions.

Main Methods:

Adaptation, sterilization, and deployment of a commercial downhole sampler (PDSshort) for deep aquifer sampling.
Comparison of cell counts and bacterial taxonomic diversity (high-throughput sequencing) between wellhead and downhole samples.
Analysis of beta-diversity to assess microbial contamination in water samples.

Main Results:

Water samples collected at the wellhead, even after extensive rinsing, contained microbial contaminants, as indicated by beta-diversity analysis.
The downhole sampler procedure was the sole method to guarantee microbiologically pure formation water samples.
The downhole sampler successfully maintained formation water and its autochthonous microbial community at *in situ* pressure.

Conclusions:

Downhole sampling is essential for ensuring the purity and representativeness of deep aquifer microbial communities.
The validated protocol and downhole sampler are critical for accurate interpretation of deep biosphere functioning.
Preserving sample integrity is paramount for reliable microbiological insights into deep subsurface environments.