The Influence of Outer Membrane Protein on Ampicillin Resistance of Vibrio parahaemolyticus

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Xiangyu Meng1, Danyang Huang2, Qing Zhou1

  • 1School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China.

The Canadian Journal of Infectious Diseases & Medical Microbiology = Journal Canadien Des Maladies Infectieuses Et De La Microbiologie Medicale
|January 23, 2023
PubMed

Insights

Outer membrane protein gene deletions in Vibrio parahaemolyticus significantly increased ampicillin resistance. This enhanced antibiotic resistance is linked to beta-lactamase expression and altered peptidoglycan synthesis pathways.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Drug Resistance

Background:

  • Antibiotic resistance in food-borne pathogens like Vibrio parahaemolyticus is a growing concern.
  • The molecular mechanisms underlying antibiotic resistance in V. parahaemolyticus are not fully understood.

Purpose of the Study:

  • To investigate the role of outer membrane proteins (OMPs) in ampicillin resistance in V. parahaemolyticus.
  • To elucidate the molecular mechanisms contributing to increased ampicillin resistance in OMP mutants.

Main Methods:

  • Seven OMP genes were individually deleted in V. parahaemolyticus ATCC33846.
  • Antibiotic resistance profiling of the resulting mutants against 14 antibiotics was performed.
  • Gene transcription levels related to cell wall synthesis and beta-lactamase activity were analyzed using RT-qPCR.

Main Results:

  • Deletion of 7 OMP genes led to significantly increased ampicillin resistance in V. parahaemolyticus mutants.
  • Increased ampicillin resistance correlated with the expression of beta-lactamase.
  • Alterations in peptidoglycan synthesis, including reduced penicillin-binding protein (PBP) transcription and changes in transpeptidase and carboxypeptidase activity, were observed.

Conclusions:

  • Outer membrane protein mutations can enhance ampicillin resistance in V. parahaemolyticus.
  • The observed resistance is associated with beta-lactamase production and modifications in peptidoglycan synthesis pathways.
  • This study offers novel insights into ampicillin resistance mechanisms in OMP mutants, highlighting the interplay with cell wall biosynthesis.

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