Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electrophoresis: Overview01:20

Electrophoresis: Overview

2.1K
Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
2.1K
Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

6.2K
Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such...
6.2K
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

481
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
481
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

296
Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
296
SDS-PAGE01:27

SDS-PAGE

28.6K
Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed  polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact...
28.6K
DNA Agarose Gel Electrophoresis02:35

DNA Agarose Gel Electrophoresis

97.9K
Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...
97.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Imaging Transgenic Nude Mice Expressing Spectrally-distinct Fluorescent Reporters Emitting From Blue to Far Red Light With One Instrument With Single-nanometer-tuning of Laser-excitation Fluorescence.

Cancer diagnosis & prognosis·2024
Same author

Non-Invasive Direct Visualization of Luciferase-Luciferin Emitted Light Producing True Images from an Orthotopic Lung Cancer Xenograft Model in Nude Mice Using an Ultra-sensitive Low-light Camera and Optics.

In vivo (Athens, Greece)·2024
Same author

Head-to-head Comparison of Green Fluorescent Protein (GFP) Imaging With Luciferase-luciferin Imaging <i>In Vivo</i> Using Single-nanometer Laser-excitation Tuning and an Ultra-low-light-detection Camera and Optics Demonstrates the Superiority of GFP.

Anticancer research·2024
Same author

Precise Non-invasive Imaging Mouse Model of Pancreatic Cancer: Very Narrow Band-width Laser Fluorescence Excitation of Green Fluorescent Protein Provides Ultra-bright Tumor Images With no Skin Autofluorescence.

Cancer diagnosis & prognosis·2024
Same author

Non-invasive Fluorescence Imaging of Breast Cancer Metastasis to the Brain in an Orthotopic Nude-mouse Model With Very-narrow-band-width Laser Excitation of Red Fluorescent Protein Resulting in an Ultra-bright Signal Without Skin Autofluorescence.

In vivo (Athens, Greece)·2023
Same author

Osteochondral allograft transplant to the medial femoral condyle using a medial or lateral femoral condyle allograft: is there a difference in graft sources?

The American journal of sports medicine·2014

Related Experiment Video

Updated: Aug 13, 2025

Electroeluting DNA Fragments
06:13

Electroeluting DNA Fragments

Published on: September 5, 2010

27.9K

Overview of Digital Electrophoresis Analysis.

Qi Wang1, Neil Chang1, Butch Moomaw2

  • 1Analytik Jena US, LLC, Upland, California.

Current Protocols
|January 23, 2023
PubMed
Summary

Digital imaging using sCMOS and CCD cameras offers rapid documentation and analysis of protein and DNA electrophoresis. This technology enables efficient archiving and detailed examination of gel electropherograms.

Keywords:
2D PAGECCDanalysiselectrophoresisimagingsCMOS

More Related Videos

Denaturing Gradient Gel Electrophoresis DGGE
10:52

Denaturing Gradient Gel Electrophoresis DGGE

Published on: February 25, 2007

21.4K
Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method
07:58

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method

Published on: September 19, 2018

6.7K

Related Experiment Videos

Last Updated: Aug 13, 2025

Electroeluting DNA Fragments
06:13

Electroeluting DNA Fragments

Published on: September 5, 2010

27.9K
Denaturing Gradient Gel Electrophoresis DGGE
10:52

Denaturing Gradient Gel Electrophoresis DGGE

Published on: February 25, 2007

21.4K
Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method
07:58

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method

Published on: September 19, 2018

6.7K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biotechnology

Background:

  • Digital imaging is essential for documenting and analyzing electrophoretic separations.
  • Traditional methods can be time-consuming and less efficient for data management.

Purpose of the Study:

  • To provide a comprehensive overview of digital imaging techniques for gel electrophoresis.
  • To define key terms, explain capture processes, and review available technologies.
  • To introduce software and methods for analyzing one- and two-dimensional digital images.

Main Methods:

  • Utilizing sCMOS and CCD-based cameras for rapid image acquisition.
  • Employing image analysis software for data processing and interpretation.
  • Reviewing various digital imaging technologies and capture processes.

Main Results:

  • Digital imaging allows for swift and accurate documentation of electrophoretic separations.
  • Image analysis software facilitates efficient archiving and detailed examination of gel electropherograms.
  • The overview covers essential terminology, capture techniques, and analytical approaches.

Conclusions:

  • Digital imaging is the preferred method for electrophoretic gel analysis.
  • The technology enhances efficiency in data acquisition, archiving, and analysis.
  • This approach supports robust documentation and detailed study of protein and DNA separations.