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Related Experiment Video

Updated: May 12, 2026

Preparation of Oligomeric β-amyloid1-42 and Induction of Synaptic Plasticity Impairment on Hippocampal Slices

Preparation of Oligomeric β-amyloid1-42 and Induction of Synaptic Plasticity Impairment on Hippocampal Slices

Published on: July 14, 2010

Soluble amyloid-β precursor peptide does not regulate GABA_B receptor activity.

Pascal Dominic Rem¹, Vita Sereikaite², Diego Fernández-Fernández¹

¹Department of Biomedicine, Pharmazentrum, University of Basel, Basel, Switzerland.

|January 23, 2023

Summary

This summary is machine-generated.

Amyloid precursor protein (APP) peptide APP17 binds GABA_B receptors (GBRs) but does not activate them. This suggests secreted APP (sAPP) influences neuronal activity via other receptors, not GBRs.

Keywords:

G protein-coupled receptors GABAB mouse neuroscience soluble amyloid-β precursor protein

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Published on: January 25, 2018

Area of Science:

Neuroscience
Molecular Biology
Cell Biology

Background:

Secreted amyloid precursor protein (sAPP) modulates neuronal activity by interacting with cell surface receptors.
Previous studies suggested sAPP and a derived peptide (APP17) bind to GABA_B receptors (GBRs) and mimic their inhibitory effects, proposing sAPP activates GBRs.

Purpose of the Study:

To investigate whether APP17 and sAPP functionally activate classical GABA_B receptor signaling pathways.
To determine if APP17 acts as a functional ligand for GBRs.

Main Methods:

Binding assays to confirm APP17 affinity for GBRs.
Biochemical and electrophysiological analyses in heterologous cells to assess GBR activity.
In vitro and in vivo experiments to evaluate APP17 effects on synaptic GBR localization, GBR-activated currents, neurotransmitter release, and neuronal activity.

Main Results:

APP17 demonstrated nanomolar affinity binding to GBRs.
Biochemical and electrophysiological studies revealed APP17 does not influence GBR activity in heterologous cells.
APP17 did not affect synaptic GBR localization, GBR-activated K+ currents, neurotransmitter release, or neuronal activity in vitro or in vivo.

Conclusions:

APP17 is not a functional ligand for GABA_B receptors.
The findings indicate that secreted APP (sAPP) exerts its neuronal effects through mechanisms independent of GABA_B receptor activation.