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Updated: Aug 13, 2025

Cytotoxicity Assays with Zebrafish Cell Lines

Cytotoxicity Assays with Zebrafish Cell Lines

Published on: January 6, 2023

Cytotoxicity Assays with Zebrafish Cell Lines.

Irisdoris Rodrigues de Souza¹, Tainá Wilke Sivek¹, Júlia Beatriz Vaz de Oliveira¹

¹Graduate Program in Genetics, Department of Genetics, Federal University of Paraná (UFPR).

Journal of Visualized Experiments : Jove

|January 23, 2023

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This study details cytotoxicity assays for fish cell lines, evaluating cell viability to predict acute toxicity. These methods offer a reliable approach for ecotoxicity testing of chemical substances.

Area of Science:

Environmental toxicology
Cell biology
Aquatic toxicology

Background:

Fish cell lines are increasingly utilized in ecotoxicity studies.
Cytotoxicity assays show promise for predicting fish acute toxicity.

Purpose of the Study:

To present modified cytotoxicity assays for evaluating cell viability in zebrafish embryo (ZEM2S) and liver (ZFL) cell lines.
To assess mitochondrial, membrane, and lysosomal integrity using specific assays.

Main Methods:

Utilized zebrafish embryo (ZEM2S) and liver (ZFL) cell lines in 96-well plates.
Evaluated cytotoxicity using Alamar Blue (AB), MTT, CFDA-AM, and Neutral Red (NR) assays.
Performed assays measuring mitochondrial integrity (AB, MTT), membrane integrity (CFDA-AM), and lysosomal integrity (NR).

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Last Updated: Aug 13, 2025

Cytotoxicity Assays with Zebrafish Cell Lines

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Published on: January 6, 2023

Acute Kidney Injury Model Induced by Cisplatin in Adult Zebrafish

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Main Results:

Developed a protocol for simultaneous AB and CFDA-AM assays, followed by NR assay on the same plate.
MTT assay was performed on a separate plate.
Readouts were obtained via fluorescence (AB, CFDA-AM) and absorbance (MTT, NR).

Conclusions:

Cytotoxicity assays using fish cell lines provide a viable method for assessing the acute toxicity of chemical substances.
These assays contribute to advancing ecotoxicity testing protocols for aquatic organisms.