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Abl2 Kinase Differentially Regulates iGluRs Current Activity and Synaptic Localization.

M Kabirova1,2, M Reichenstein1,3,4, N Borovok3

  • 1Department of Molecular Biology, Faculty of Natural Sciences, Ariel University, 4070000, Ariel, Israel.

Cellular and Molecular Neurobiology
|January 23, 2023
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Summary
This summary is machine-generated.

Abelson kinase 2 (Abl2) reduces excitatory synaptic currents but stabilizes dendritic spines. This suggests Abl2 balances synaptic transmission and structural integrity in mature neurons.

Keywords:
AMPAAbelson non-receptor tyrosine kinase (Abl)Miniature excitatory post synaptic currents (mEPSC)NMDASpontaneous synaptic activity

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Area of Science:

  • Neuroscience
  • Cell Biology
  • Molecular Biology

Background:

  • Abelson non-receptor tyrosine kinases (Abl1 and Abl2) are crucial for neuronal development and synaptic function.
  • Abl2 is known to be essential for optimal synaptic functioning in mature neurons.
  • Previous work showed Abl1 at presynapses and Abl2 at postsynapses, modulating synaptic transmission.

Purpose of the Study:

  • To investigate the specific role of Abl2 in regulating the postsynaptic component of miniature excitatory postsynaptic currents (mEPSCs).
  • To understand how Abl2 activity influences AMPA and NMDA receptor-mediated currents.
  • To explore the impact of Abl2 on dendritic spine stability and synapse maintenance.

Main Methods:

  • Electrophysiological recordings of mEPSCs in neurons.
  • Manipulation of Abl2 activity (acute and prolonged activation).
  • Analysis of AMPA and NMDA receptor current amplitudes.
  • Assessment of dendritic spine morphology and synapse density.

Main Results:

  • Abl2 activation reduced mEPSC amplitude, as well as AMPA and NMDA current amplitudes.
  • Prolonged Abl2 activity led to stabilization of dendritic spines.
  • Abl2 activity contributed to maintaining active synapses on distal dendrites.

Conclusions:

  • Abl2 activation attenuates ion currents through ionotropic glutamatergic receptors, potentially by altering receptor sensitivity or channel gating.
  • Despite reducing synaptic currents, Abl2 plays a vital role in stabilizing dendritic spines and maintaining synapses, particularly on distal dendrites.
  • Abl2 expression influences excitatory synapse density, while its activity affects receptor-mediated current density.