Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Aphid effector Mp10 balances immune suppression and defence activation through EDS1-dependent modulation of plant DAMP responses.

The New phytologist·2025

Same author

Molecular Basis of Eusocial Complexity: The Case of Worker Reproductivity in Bees.

Genome biology and evolution·2024

Same author

Developmental Diet Alters the Fecundity-Longevity Relationship and Age-Related Gene Expression in Drosophila melanogaster.

The journals of gerontology. Series A, Biological sciences and medical sciences·2023

Same author

Costs of reproduction are present but latent in eusocial bumblebee queens.

BMC biology·2023

Same author

Gene expression during larval caste determination and differentiation in intermediately eusocial bumblebees, and a comparative analysis with advanced eusocial honeybees.

Molecular ecology·2020

Same author

Transgressive segregation reveals mechanisms of <i>Arabidopsis</i> immunity to <i>Brassica</i>-infecting races of white rust (<i>Albugo candida</i>).

Proceedings of the National Academy of Sciences of the United States of America·2019

Search research articles

Related Experiment Video

Updated: Aug 13, 2025

RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs

RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs

Published on: July 11, 2020

Detection of miRNAs.

Afrah Bawazeer¹, David C Prince²

¹School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich, UK.

Methods in Molecular Biology (Clifton, N.J.)

|January 23, 2023

Summary

This summary is machine-generated.

Detecting microRNAs (miRNAs), crucial gene regulators, is difficult due to their small size. Current methods offer solutions, but further innovation is needed for easier miRNA detection, especially for disease biomarkers.

Keywords:

Biomarkers MiRNA detection Point-of-care setting

More Related Videos

Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay

Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay

Published on: May 25, 2015

Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer

Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer

Published on: March 14, 2019

Related Experiment Videos

Last Updated: Aug 13, 2025

RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs

RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs

Published on: July 11, 2020

Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay

Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay

Published on: May 25, 2015

Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer

Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer

Published on: March 14, 2019

Area of Science:

Molecular Biology
Biochemistry
Genetics

Background:

MicroRNAs (miRNAs) are small, noncoding RNA molecules that regulate gene expression.
miRNAs are implicated in numerous biological processes and human diseases.
Detecting miRNAs is challenging due to their short sequences and low abundance.

Purpose of the Study:

To review conventional and emerging techniques for miRNA detection.
To discuss the application of these techniques in point-of-care (POC) settings for disease biomarker detection.
To identify areas for future innovation in miRNA detection methodologies.

Main Methods:

Review of existing literature on miRNA detection techniques.
Analysis of conventional methods (Northern blot, microarray, RT-qPCR).
Evaluation of emerging methods (nanomaterials, electrochemical assays).

Main Results:

Various techniques exist for miRNA detection, each with advantages and limitations.
Combinations of techniques are being explored for POC applications.
Current methods still present challenges for sensitive and efficient miRNA detection.

Conclusions:

Despite advancements, improved methods are needed for robust miRNA detection.
Further innovation can enhance the utility of miRNAs as disease biomarkers.
Development of novel detection strategies is crucial for clinical applications.