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Related Experiment Video

Updated: Aug 13, 2025

In ovo Expression of MicroRNA in Ventral Chick Midbrain
09:19

In ovo Expression of MicroRNA in Ventral Chick Midbrain

Published on: September 16, 2013

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Determining miRNA Expression Patterns in Xenopus.

Marco Antonaci1, Alice M Godden1, Grant N Wheeler2

  • 1School of Biological Sciences, University of East Anglia, Norwich, UK.

Methods in Molecular Biology (Clifton, N.J.)
|January 23, 2023
PubMed
Summary

This chapter details visualizing microRNAs (miRNAs) in Xenopus embryos using whole-mount in situ hybridization (WISH). Two methods, LNA-WISH and pri-miRNA-WISH, are presented for mature and immature miRNA visualization, respectively.

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Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Genetics

Background:

  • Whole-mount in situ hybridization (WISH) allows RNA localization in embryos.
  • MicroRNAs (miRNAs) are key regulators of gene expression.
  • Visualizing miRNAs in vivo is crucial for understanding their function.

Purpose of the Study:

  • To describe methods for visualizing miRNAs in Xenopus embryos.
  • To present two distinct WISH approaches for miRNA detection.

Main Methods:

  • Whole-mount in situ hybridization (WISH) using labeled probes.
  • Locked nucleic acid (LNA)-based WISH (LNA-WISH) for mature miRNAs.
  • Primary miRNA (pri-miRNA)-WISH for immature miRNAs.

Main Results:

Keywords:
DevelopmentExpression patternWISHXenopusmiRNA

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Last Updated: Aug 13, 2025

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  • Successful visualization of mature miRNAs in Xenopus embryos using LNA-WISH.
  • Successful visualization of pri-miRNAs in Xenopus embryos using pri-miRNA-WISH.
  • Demonstration of spatial and temporal miRNA expression patterns.

Conclusions:

  • LNA-WISH and pri-miRNA-WISH are effective for visualizing different miRNA forms in Xenopus.
  • These techniques provide insights into miRNA-mediated gene regulation during development.
  • The described methods are valuable tools for developmental and molecular biologists.