A robust high selectivity fluorescence turn-on nanoprobe for peroxynitrite detection in inflammatory cells and mice

Changed levels of intracellular peroxynitrite anion (ONOO^-) are closely related to the occurrence and development of inflammation. Specific imaging of ONOO^- at sites of inflammation can be of great significance not only for inflammation diagnosis but also for obtaining a deeper understanding of the role of ONOO^- in inflammation. Therefore, there is an urgent need for constructing some reliable tools to study the relationship between ONOO^- and inflammation in biosystems. In this work, we developed a robust high selectivity fluorescence turn-on nanoprobe (Rhb-ONOO) for inflammation-targeted imaging of ONOO^-. The Rhb-ONOO was obtained by self-assembly of amphiphilic Rhb-ONOO, which was constructed by the condensation reaction of the hydrophobic, ONOO^--response and deep red-emitting fluorophore (Rhb) with hydrophilic biopolymer glycol chitosan (GC). Rhb-ONOO showed rapid response towards ONOO^- during 60 s, high sensitivity with 19-fold enhancement of fluorescence intensity ratio (I₆₂₈/I₀), and excellent selectivity towards ONOO^- over other analytes as well as a good linear relationship was observed between the I₆₂₈/I₀ and the ONOO^- concentration range 0-1 μM, with an excellent limit of detection (LOD) of 33 nM. Impressively, it was successfully employed Rhb-ONOO for ONOO^- imaging in living inflammatory cells and drug-induced inflammatory mice, illustrating nanoprobe Rhb-ONOO has excellent potential for further study ONOO^--related inflammatory diseases.