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Related Experiment Video

Updated: Aug 13, 2025

Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX

Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX

Published on: June 27, 2017

T7Max transcription system.

Christopher Deich¹, Brock Cash¹, Wakana Sato¹

¹Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN, USA.

Journal of Biological Engineering

|January 23, 2023

Summary

This summary is machine-generated.

A modified T7 promoter, T7Max, significantly boosts protein expression yields in cell-free systems. This breakthrough enhances gene expression from linear DNA templates without requiring protein engineering.

Keywords:

cell-free protein expression in vitro transcription in vitro translation synthetic cells

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Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX

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Rapid Characterization of Genetic Parts with Cell-Free Systems

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Published on: August 30, 2021

Area of Science:

Molecular Biology
Biotechnology

Background:

Cell-free protein expression from linear DNA templates faces challenges like template degradation and lower transcription yields compared to live cells.
Current in vitro translation systems commonly use T7 RNA polymerase.

Purpose of the Study:

To characterize a novel T7 RNA polymerase promoter variant designed to increase gene expression yields in in vitro systems.
To evaluate the efficacy of this modified promoter in various cell-free protein expression systems and with linear DNA templates.

Main Methods:

Characterization of a modified T7 RNA polymerase promoter (T7Max).
Testing T7Max in common in vitro protein expression systems.
Assessing protein expression yields from linear DNA templates driven by T7Max.

Main Results:

The T7Max promoter significantly increases protein expression yields in various in vitro systems.
T7Max enables enhanced protein expression from linear DNA templates.
The modified promoter functions with standard T7 RNA polymerase.

Conclusions:

The T7Max promoter offers a method to improve yields in T7 RNA polymerase-based cell-free expression systems.
No protein engineering is required to utilize the T7Max promoter.
This technique is broadly applicable to existing T7 RNA polymerase-based expression platforms.