Visual Electrochemiluminescence Biosensor Chip Based on Distance Readout for Deoxynivalenol Detection

Area of Science:

• Biosensing
• Electrochemistry
• Nanomaterials

Background:

• Visual electrochemiluminescence (ECL) biosensors offer portable and low-cost detection.
• Traditional visual ECL sensors face limitations due to intensity-based detection, which is susceptible to environmental interference.

Purpose of the Study:

• To develop a novel visual ECL biosensor chip utilizing a distance readout strategy for improved detection accuracy.
• To enhance the ECL signal and enable quantification based on a measurable distance rather than intensity.

Main Methods:

• Fabrication of a biosensor chip with distinct detection and visual channel regions modified with graphene oxide (GO) and gold nanoparticles (AuNPs)@Ti3C2 nanocomposites.
• Utilizing aptamer release from GO to alter electrode potential in the visual channel, generating ECL signals over a specific distance.
• Employing AuNPs@Ti3C2 nanocomposites to amplify ECL intensity.

Main Results:

• The developed biosensor chip successfully implements a distance readout for visual ECL sensing.
• The incorporation of AuNPs@Ti3C2 nanocomposites resulted in a six-fold enhancement of ECL intensity.
• The sensor achieved quantitative detection of deoxynivalenol toxin in real samples based on the length of the ECL signal.

Conclusions:

• A novel visual ECL biosensor with a distance readout strategy has been successfully developed.
• This approach overcomes the limitations of intensity-based detection, offering enhanced stability and accuracy.
• The biosensor demonstrates significant potential for on-site, portable, and cost-effective toxin detection.