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Updated: Aug 13, 2025

Detection of Protease Activity by Fluorescent Peptide Zymography
Published on: January 20, 2019
Peptomer substrates for quantitative pattern-recognition sensing of proteases
Mariah J Austin1, Hattie C Schunk1,2, Natalie Ling1
1McKetta Department of Chemical Engineering, University of Texas at Austin, Austin, TX, 78712, USA. arosales@che.utexas.edu.
Abstract:
The utility of active proteases as biomarkers is often limited by overlapping substrate specificity. Here, this feature is leveraged to develop a quantitative pattern-recognition sensing system driven by the degradation patterns of peptide-peptoid hybrid substrates to classify proteases and estimate their concentration by multivariate data analysis.
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