Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Concurrent control of natural and robotic limbs through a tactile-encoded brain-computer interface.

Nature communications·2026

Same author

Jasmonate, salicylate, and ethylene-responsive transcriptomics discovery in spikelets of three wheat genotypes reveals a rapid and conserved response for jasmonate signaling.

Plant signaling & behavior·2026

Same author

Assessing different Protein A resins' homodimer separation potentials through processing a two-antibody-containing artificial mixture.

Protein expression and purification·2026

Same author

Reply to "Letter to the editor: methodological considerations in the benchmarking of AI-based protein-aptamer complex prediction".

Briefings in bioinformatics·2026

Same author

Multi-Omics Data Integration Clustering for Cancer Subtypes Identification Based on Motif High-Order Similarity Graph and Tensor Regularization.

Genes·2026

Same author

EEG Ensemble Learning Framework for Prognostic Prediction of Post-rTMS Motor Recovery in Stroke.

IEEE transactions on bio-medical engineering·2026

Same journal

Discovery of a potent sGC stimulator with once-daily dosing potential for the treatment of hypertension.

Bioorganic & medicinal chemistry letters·2026

Same journal

OracleScreen-LILRB4 (HTS-Oracle v3): machine learning-guided discovery of myeloid immune checkpoint binders validated in patient-derived cells.

Bioorganic & medicinal chemistry letters·2026

Same journal

Rational design and N-terminal acylation of a lactam-cyclized 11-residue antimicrobial peptide for improved antibacterial potency.

Bioorganic & medicinal chemistry letters·2026

Same journal

Rational design, synthesis, and biological evaluation of chalcone hybrids including benzoylpiperazin (phenylacetylpiperazin)-thiophene as anti- Alzheimer's agents.

Bioorganic & medicinal chemistry letters·2026

Same journal

New aminopiperidine-azoles as antifungal agents against Candida albicans with mechanistic insights into the cAMP-PKA pathway.

Bioorganic & medicinal chemistry letters·2026

Same journal

Novel 1H-pyrrole-2,5-dione derivatives as potential anti-diabetic agents: design, synthesis, in vitro, in ovo, and molecular docking studies.

Bioorganic & medicinal chemistry letters·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Aug 12, 2025

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Published on: January 7, 2017

Thermal treatment affects aptamers' structural profiles.

Hayley-Ann Bennett¹, Yifeng Li², Hongbin Yan¹

¹Department of Chemistry and Centre for Biotechnology, Brock University, 1812 Sir Isaac Brock Way, St. Catharines, ON L2S 3A1, Canada.

Bioorganic & Medicinal Chemistry Letters

|January 24, 2023

Summary

This summary is machine-generated.

Thermal annealing and sonication can significantly alter aptamer structures, leading to a more consistent conformational state. This optimization is crucial for improving aptamer selection and result interpretation in molecular biology applications.

Keywords:

Anion exchange HPLC Aptamer Circular dichroism Conformational plasticity Secondary structure Thermal anneal

More Related Videos

Primer-Free Aptamer Selection Using A Random DNA Library

Primer-Free Aptamer Selection Using A Random DNA Library

Published on: July 26, 2010

Determining the Thermodynamic and Kinetic Association of a DNA Aptamer and Tetracycline Using Isothermal Titration Calorimetry

Determining the Thermodynamic and Kinetic Association of a DNA Aptamer and Tetracycline Using Isothermal Titration Calorimetry

Published on: August 23, 2022

Related Experiment Videos

Last Updated: Aug 12, 2025

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Published on: January 7, 2017

Primer-Free Aptamer Selection Using A Random DNA Library

Primer-Free Aptamer Selection Using A Random DNA Library

Published on: July 26, 2010

Determining the Thermodynamic and Kinetic Association of a DNA Aptamer and Tetracycline Using Isothermal Titration Calorimetry

Determining the Thermodynamic and Kinetic Association of a DNA Aptamer and Tetracycline Using Isothermal Titration Calorimetry

Published on: August 23, 2022

Area of Science:

Biochemistry
Molecular Biology
Analytical Chemistry

Background:

Aptamers are nucleic acid-based ligands with high specificity.
Understanding aptamer conformational flexibility is key to their application.
Non-denaturing chromatography is a powerful tool for studying biomolecular structures.

Purpose of the Study:

To investigate the conformational flexibility of adenosine, ampicillin, and quinine aptamers.
To determine the effects of thermal annealing and sonication on aptamer structures.
To correlate structural changes with analytical data from spectroscopy and chromatography.

Main Methods:

Anion-exchange high-performance liquid chromatography (HPLC) under non-denaturing conditions.
Thermal annealing (heating followed by slow cooling).
UV/Vis spectroscopy, circular dichroism (CD) spectroscopy, and melt curve analysis.

Main Results:

Aptamers existed in multiple conformational states without thermal treatment.
Thermal annealing induced a predominant single structure (likely partial hairpin) in all studied aptamers.
Sonication resulted in a single eluting species for quinine aptamer, but not adenosine or ampicillin aptamers.
Spectroscopic and melt curve data confirmed structural changes induced by annealing and sonication.

Conclusions:

Thermal annealing is effective in achieving a homogeneous aptamer conformation.
Sonication can also induce conformational homogeneity, but aptamer-specifically.
These findings support optimizing aptamer preparation for enhanced selection efficiency and consistent experimental outcomes.