Ca²⁺ deficiency triggers panicle degeneration in rice mediated by Ca²⁺ /H⁺ exchanger OsCAX1a

Increasing rice yield has always been one of the primary objectives of rice breeding. However, panicle degeneration often occurs in rice-growing regions and severely curbs rice yield. In this study, we obtained a new apical panicle degeneration mutant, which induces a marked degeneration rate and diminishes the final grain yield. Cellular and physiological analyses revealed that the apical panicle undergoes programmed cell death, accompanied by excessive accumulations of peroxides. Following, the panicle degeneration gene OsCAX1a was identified in the mutant, which was involved in Ca²⁺ transport. Hydroponics assays and Ca²⁺ quantification confirmed that Ca²⁺ transport and distribution to apical tissues were restricted and over-accumulated in the mutant sheath. Ca²⁺ transport between cytoplasm and vacuole was affected, and the reduced Ca²⁺ content in the vacuole and cell wall of the apical panicle and the decreased Ca²⁺ absorption appeared in the mutant. RNA-Seq data indicated that the abnormal CBL (calcineurin b-like proteins) pathway mediated by deficient Ca²⁺ might occur in the mutant, resulting in the burst of ROS and programmed cell death in panicles. Our results explained the key role of OsCAX1a in Ca²⁺ transport and distribution and laid a foundation to further explore the genetic and molecular mechanisms of panicle degeneration in rice.