Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Human Amniotic Membrane as an Interpositional Material in Temporomandibular Joint Arthroplasty for Intraarticular Pain and Dysfunction: A Randomized Clinical Trial.

Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons·2026
Same author

Cryopreserved human amnion in pediatric burn care: A retrospective single-center study over a decade.

Burns : journal of the International Society for Burn Injuries·2026
Same author

Results of adjunctive homogenate amniotic membrane eye drops in the management of refractory ocular surface disease with dry eye manifestations.

International ophthalmology·2026
Same author

Cryopreservation preserves the morpho-structural and mechanical integrity of human decellularized tracheas and supports a reduced immunogenic profile.

Frontiers in bioengineering and biotechnology·2026
Same author

Geometric Accuracy of 3D-Printed Composite Dental Restorations Compared with the Original STL Design.

Journal of functional biomaterials·2026
Same author

Aortic homografts for native and prosthetic aortic valve and root endocarditis: Results from the EUropean REgistry of Cryopreserved Aortic Homografts EURECAH.

The Journal of thoracic and cardiovascular surgery·2026

Related Experiment Video

Updated: Aug 12, 2025

Procedure for Decellularization of Porcine Heart by Retrograde Coronary Perfusion
11:30

Procedure for Decellularization of Porcine Heart by Retrograde Coronary Perfusion

Published on: December 6, 2012

24.0K

Decellularized cryopreserved human pericardium: a validation study towards tissue bank practice.

Giulia Montagner1, Antonia Barbazza2, Andrea Tancredi Lugas3,4

  • 1Fondazione Banca Dei Tessuti del Veneto Onlus, Treviso, Italy. gmontagner@fbtv-treviso.org.

Cell and Tissue Banking
|January 25, 2023
PubMed
Summary

This study presents a new decellularization method for homologous pericardium, preserving its structure for cardiac septal defect repair. This approach offers a promising alternative to synthetic materials, reducing surgical complications.

Keywords:
AllograftCardiac septal defectsDecellularizationPericardiumTissue bank

More Related Videos

Decellularization of Whole Human Heart Inside a Pressurized Pouch in an Inverted Orientation
06:28

Decellularization of Whole Human Heart Inside a Pressurized Pouch in an Inverted Orientation

Published on: November 26, 2018

11.4K
Comparable Decellularization of Fetal and Adult Cardiac Tissue Explants as 3D-like Platforms for In Vitro Studies
08:10

Comparable Decellularization of Fetal and Adult Cardiac Tissue Explants as 3D-like Platforms for In Vitro Studies

Published on: March 21, 2019

7.0K

Related Experiment Videos

Last Updated: Aug 12, 2025

Procedure for Decellularization of Porcine Heart by Retrograde Coronary Perfusion
11:30

Procedure for Decellularization of Porcine Heart by Retrograde Coronary Perfusion

Published on: December 6, 2012

24.0K
Decellularization of Whole Human Heart Inside a Pressurized Pouch in an Inverted Orientation
06:28

Decellularization of Whole Human Heart Inside a Pressurized Pouch in an Inverted Orientation

Published on: November 26, 2018

11.4K
Comparable Decellularization of Fetal and Adult Cardiac Tissue Explants as 3D-like Platforms for In Vitro Studies
08:10

Comparable Decellularization of Fetal and Adult Cardiac Tissue Explants as 3D-like Platforms for In Vitro Studies

Published on: March 21, 2019

7.0K

Area of Science:

  • Biomaterials Science
  • Cardiovascular Surgery
  • Tissue Engineering

Background:

  • Pericardial patches are used for cardiac septal defect repair.
  • Current alternatives include autologous, synthetic, or xenograft materials.
  • Allogenic decellularized pericardium offers potential to reduce immunogenicity and complications.

Purpose of the Study:

  • To evaluate a novel decellularization protocol for homologous pericardium.
  • To assess the efficacy of the protocol after cryopreservation.
  • To determine suitability as a tissue bank procedure for congenital heart disease repair.

Main Methods:

  • Development of a new decellularization protocol for human pericardium.
  • Evaluation of the protocol's effectiveness in removing cellular and nucleic acid content.
  • Assessment of preserved collagen and biomechanical properties post-decellularization and cryopreservation.

Main Results:

  • The decellularization technique successfully removed cells and nucleic acids.
  • Collagen structure and biomechanical properties of the pericardium were preserved.
  • The protocol is suitable for tissue banking and cryopreservation.

Conclusions:

  • The novel decellularization protocol is effective for homologous pericardium.
  • This method yields a promising graft material for congenital heart defect repair.
  • Preserved biomechanical integrity and reduced immunogenicity make it a viable alternative.