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A smart ratiometric fluoresence and colorimetry dual-responsive sensor for morin determination based on the complex
Xin-He Duan1, Hong-Wei Li1, Yuqing Wu1
1State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, No. 2699 Qianjin Street, Changchun, 130012, PR China; Institute of Theoretical Chemistry, College of Chemistry, Jilin University, No. 2 Liutiao Road, Changchun, 130023, PR China.
Abstract:
As a representative flavonoid, morin exhibits multi-biological activities, but its abuse endangers human health. Developing advanced technology for morin determination is urgently needed. In this study, a dual-responsive approach was reported for morin based on the complexing of carbon quantum dots (CQDs) and polyethyleneimine (PEI). The CQDs were fabricated via an improved hydrothermal method employing tyrosine and malic acid. Binding with PEI induced an 8-fold emission enhancement and a slight red-shift to 445 nm of CQDs because of the complexing of PEI and CQDs. Further morin introduction led to the blue emission (445 nm) quenching of CQDs-PEI and a yellow emission (560 nm) generation, which contributed a ratiometric fluorescence approach for morin determination between 2.0 and 32 μM, with a limit of detection (LOD) of 45 nM. Meanwhile, under sunlight the color of CQDs-PEI became yellow upon morin addition, which developed a colorimetric method for morin determination in a wide range between 2.0 and 100 μM (LOD = 69 nM). The developed dual-responsive method either displayed accurate results for morin in diluted human and bovine serum, being potential for actual sample analysis. Finally, a visual detection based on the smartphone was constructed and applied for the real-time determination of morin.
