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Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
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Updated: Aug 12, 2025

An In Vitro Assay to Study Platelet Migration Using RGD-Functionalized Avidin-Biotin Tethers

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Published on: November 8, 2024

Biotinylated Platelets: A Promising Labeling Technique?

Stefan F van Wonderen¹, Floor L F van Baarle¹, Sanne de Bruin¹

¹Amsterdam UMC location University of Amsterdam, Department of Intensive Care and Laboratory of Experimental Intensive Care and Anesthesiology, Meibergdreef 9, Amsterdam, the Netherlands.

Transfusion Medicine Reviews

|January 25, 2023

Summary

Biotinylation offers a safe and effective alternative to radioactive labeling for tracking transfused platelets (PLTs) in research. This method allows for in vivo monitoring and isolation of biotin-labeled platelets (bioPLTs) with minimal activation.

Keywords:

Biotin Blood donation Labeling Platelet Platelet survival Thrombocytopenia Transfusion

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Last Updated: Aug 12, 2025

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Published on: November 8, 2024

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Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics

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Published on: June 23, 2022

Area of Science:

Hematology
Biotechnology
Immunology

Background:

Platelet (PLT) labeling is crucial for assessing transfused PLT recovery and survival in vivo.
Radioactive labeling, the current gold standard, has limitations.
Biotinylation presents a promising alternative for PLT research.

Approach:

This review summarizes key publications on biotin-labeled platelets (bioPLTs).
Studies evaluated the feasibility of bioPLT transfusion in humans.
Protocols for standardized, reproducible biotinylation and non-washing methods were developed.
Preclinical studies explored using varying biotin densities for concurrent monitoring of multiple PLT populations.

Key Points:

Transfusion of bioPLTs in human recipients is feasible.
Standardized biotinylation protocols reduce PLT activation markers.
Non-washing biotin labeling methods are suitable for blood bank settings.
Multiple human PLT populations can be monitored simultaneously in vivo using different biotin densities.

Conclusions:

Biotin-labeled platelets (bioPLTs) are a viable option for human research.
BioPLTs can be safely administered, particularly at low biotin densities.
Further research supports the advancement of bioPLTs as a safer alternative to radioactive labeling.