Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Proto-oncogene expression in human normal bone marrow.

M J Evinger-Hodges1, K A Dicke, J U Gutterman

  • 1Department of Hematology, U.T. M.D. Anderson Hospital & Tumor Institute, Houston 77030.

Leukemia
|August 1, 1987
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A plant triterpenoid, avicin D, induces autophagy by activation of AMP-activated protein kinase.

Cell death and differentiation·2007
Same author

Avicins, a family of triterpenoid saponins from Acacia victoriae (Bentham), suppress H-ras mutations and aneuploidy in a murine skin carcinogenesis model.

Proceedings of the National Academy of Sciences of the United States of America·2001
Same author

Avicins, a family of triterpenoid saponins from Acacia victoriae (Bentham), inhibit activation of nuclear factor-kappaB by inhibiting both its nuclear localization and ability to bind DNA.

Proceedings of the National Academy of Sciences of the United States of America·2001
Same author

Triterpenoid saponins from Acacia victoriae (Bentham) decrease tumor cell proliferation and induce apoptosis.

Cancer research·2001
Same author

Phase I trial of the angiogenesis inhibitor TNP-470 for progressive androgen-independent prostate cancer.

Clinical cancer research : an official journal of the American Association for Cancer Research·2001
Same author

Avicins: triterpenoid saponins from Acacia victoriae (Bentham) induce apoptosis by mitochondrial perturbation.

Proceedings of the National Academy of Sciences of the United States of America·2001
Same journal

Linperlisib enhances MUC1-Tn CAR T cell efficacy by inhibiting EGR1/DUSP2 axis to prevent CAR T cell exhaustion.

Leukemia·2026
Same journal

CD7 chimeric antigen receptor T cells in patients with relapsed or refractory CD7-positive acute myeloid leukemia.

Leukemia·2026
Same journal

Single-cell architecture of purinergic signaling in human cord blood hematopoietic stem and progenitor cells.

Leukemia·2026
Same journal

Feasibility and safety of rapid glofitamab ramp-up.

Leukemia·2026
Same journal

Single-cell DNA methylation analysis uncovers epigenetic pathways in the transformation of MDS to AML.

Leukemia·2026
Same journal

PD-L2 is associated with lineage-related transcriptional programs distinct from PD-L1 in primary mediastinal large B-cell lymphoma.

Leukemia·2026
See all related articles

This study compared oncogene expression in normal and leukemia cells. Most oncogenes showed low expression in normal bone marrow, contrasting with higher levels in leukemia, indicating altered expression contributes to the leukemic state.

Area of Science:

  • Hematology
  • Molecular Biology
  • Oncology

Background:

  • Previous studies on oncogene expression in human leukemia lacked data on normal hematopoietic cells.
  • Understanding oncogene expression in normal cells is crucial for deciphering their role in leukemia.

Purpose of the Study:

  • To analyze transcript size and expression levels of six oncogenes in normal hematopoietic cells.
  • To compare these findings with those in various leukemia types (AML, ALL, CML).
  • To determine if aberrant transcript size or altered expression levels are characteristic of leukemia.

Main Methods:

  • Analysis of transcript size and expression levels of c-myc, c-myb, c-fes, c-raf, c-fos, and c-sis.
  • Comparison of fresh hematopoietic cells from normal individuals and leukemia patients.

Related Experiment Videos

  • Utilized techniques to quantify gene expression and analyze transcript sizes.

    • Main Results:

    • Low expression of c-myc, c-myb, c-fes, and c-raf in normal bone marrow versus high levels in some leukemias.
    • High expression of c-fos in both normal bone marrow and certain leukemias.
    • No detectable c-sis expression in normal samples.
    • No significant variation in transcript size for most oncogenes between normal and leukemic samples, except for c-fes.
    • Significant variations in oncogene expression levels between normal hematopoietic cells and leukemia, and among different leukemia types.

    Conclusions:

    • Aberrant transcript size is not a common feature for the studied oncogenes in leukemia.
    • Altered expression levels of proto-oncogenes are a key difference between normal hematopoietic cells and leukemia.
    • These findings provide a baseline for understanding oncogene dysregulation in leukemogenesis.