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Quantitative proteomic analysis reveals the effects of mu opioid agonists on HT22 cells

Xutong Zhang1, Yani Lou1, Dongxu Zheng1

  • 1Department of Anesthesiology, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, China.

Frontiers in Pharmacology
|January 26, 2023
PubMed

Insights

Mu opioid receptor activation by loperamide alters protein expression in nerve cells. This research sheds light on potential neurotoxic effects of opioid drugs, improving understanding of their impact on the nervous system.

Area of Science:

  • Neuroscience
  • Pharmacology
  • Proteomics

Background:

  • The mu opioid receptor is crucial in anesthesiology.
  • The neurotoxic effects of mu opioid receptor agonists are not fully understood.

Purpose of the Study:

  • To investigate the impact of mu opioid receptor agonism on cellular proteomes.
  • To identify specific protein expression changes induced by loperamide in HT22 cells.

Main Methods:

  • Utilized stable isotope labeling of amino acids in cell culture (SILAC) for quantitative proteomics.
  • Employed immobilized metal affinity chromatography (IMAC) for protein enrichment.
  • Applied high-resolution liquid chromatography-tandem mass spectrometry (LC-MS/MS) for protein identification.

Main Results:

  • Identified a total of 7,823 proteins in HT22 cells.
  • Bioinformatic analysis revealed significant alterations in cellular proteomes due to mu opioid receptor agonism.
  • Distinct changes in protein expression patterns were observed.

Conclusions:

  • Mu opioid receptor activation significantly impacts cellular protein expression.
  • These findings contribute to understanding the neurobiological effects of opioid drugs.
  • Provides insights into the potential neurotoxicity associated with mu opioid receptor agonists.
Keywords:
HT22 cellLC-MS/MSloperamidemu opioid agonistnerve injuryproteomic

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