Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cholesterol autoxidation in phospholipid membrane bilayers.

A Sevanian1, L L McLeod

  • 1Institute for Toxicology, University of Southern California, School of Pharmacy, Los Angeles 90033.

Lipids
|September 1, 1987
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Nitric oxide, cholesterol oxides and endothelium-dependent vasodilation in plasma of patients with essential hypertension.

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas·2002
Same author

Cholesterol oxidation products and fibrogenesis.

BioFactors (Oxford, England)·2002
Same author

Estrogen in the prevention of atherosclerosis. A randomized, double-blind, placebo-controlled trial.

Annals of internal medicine·2001
Same author

Up-regulation of the fibrogenic cytokine TGF-beta1 by oxysterols: a mechanistic link between cholesterol and atherosclerosis.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2001
Same author

Loss of apoB-100 secondary structure and conformation in hydroperoxide rich, electronegative LDL(-).

Free radical biology & medicine·2001
Same author

Hypochlorite induces the formation of LDL(-), a potentially atherogenic low density lipoprotein subspecies.

FEBS letters·2001

Cholesterol oxidation products in liposomes vary based on lipid composition and oxidation method. Unsaturated phospholipids promote cholesterol oxidation, with distinct product profiles under different peroxidation conditions.

Area of Science:

  • Biochemistry
  • Lipid Chemistry
  • Oxidative Stress

Background:

  • Lipid peroxidation is a key process in oxidative damage.
  • Cholesterol oxidation products (COPs) are markers of oxidative stress.
  • Understanding COP formation is crucial for biological membrane studies.

Purpose of the Study:

  • To investigate cholesterol oxidation product formation in liposomes.
  • To correlate COP levels with lipid peroxidation under various conditions.
  • To model cholesterol autoxidation in biological membranes.

Main Methods:

  • Monitoring lipid peroxidation in unilamellar liposomes.
  • Inducing peroxidation via autoxidation, superoxide radicals, gamma-irradiation, or cumene hydroperoxide.
  • Analyzing cholesterol oxidation products using chromatography and mass spectrometry.

Related Experiment Videos

Main Results:

  • Major COPs identified: 7-keto-cholesterol, cholesterol epoxides, 7-hydroperoxides, and cholestane diols.
  • Cholesterol oxidation was highest in liposomes with high unsaturated phospholipid to cholesterol ratios (4:1).
  • Oxyradical and cumene hydroperoxide peroxidation yielded distinct COP profiles.

Conclusions:

  • Liposome composition significantly influences COP formation.
  • Peroxide-dependent propagation reactions contribute to COP generation in biomembranes.
  • Liposome oxidation models provide insights into cholesterol autoxidation in biological systems.