Related Experiment Video
Updated: Aug 12, 2025
Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
Published on: April 11, 2016
SangeR: the high-throughput Sanger sequencing analysis pipeline
Kai Schmid1, Hildegard Dohmen1, Nadja Ritschel1
1Department of Neuropathology, Justus-Liebig University Giessen, Arndtstr, 16 D-35392 Giessen, Germany.
Summary:
In the era of next generation sequencing and beyond, the Sanger technique is still widely used for variant verification of inconclusive or ambiguous high-throughput sequencing results or as a low-cost molecular genetical analysis tool for single targets in many fields of study. Many analysis steps need time-consuming manual intervention. Therefore, we present here a pipeline-capable high-throughput solution with an optional Shiny web interface, that provides a binary mutation decision of hotspots together with plotted chromatograms including annotations via flat files.
Availability And Implementation:
SangeR is freely available at https://github.com/Neuropathology-Giessen/SangeR and https://hub.docker.com/repository/docker/kaischmid/sange_r.
Contact:
Kai.Schmid@patho.med.uni-giessen.de or Daniel.Amsel@patho.med.uni-giessen.de.
Supplementary Information:
Supplementary data are available at Bioinformatics online.
Related Concept Videos
03:21RNA-seq
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
01:57Sanger Sequencing