Related Experiment Video

Updated: Aug 12, 2025

Induction of Atherosclerotic Plaques Through Activation of Mineralocorticoid Receptors in Apolipoprotein E-deficient Mice
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Induction of Atherosclerotic Plaques Through Activation of Mineralocorticoid Receptors in Apolipoprotein E-deficient Mice

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Icariin alleviates atherosclerosis by regulating the miR-205-5p/ERBB4/AKT signaling pathway

Peng Huang1, Fengjun Wang2, Yibing Zhang3

  • 1Department of Experimental Pharmacology and Toxicology, School of Pharmacy, Jilin University, Changchun, China.

International Immunopharmacology
|January 26, 2023
PubMed

Insights

Icariin (ICA) effectively treats atherosclerosis (AS) by reducing lipid accumulation and plaque formation. It also inhibits vascular smooth muscle cell proliferation and migration, potentially via miR-205-5p.

Area of Science:

  • Cardiovascular Research
  • Molecular Biology
  • Pharmacology

Background:

  • Atherosclerosis (AS) poses a significant global health risk.
  • Understanding therapeutic mechanisms for AS is crucial.

Purpose of the Study:

  • To investigate the therapeutic effects of icariin (ICA) on atherosclerosis.
  • To elucidate the underlying molecular mechanisms of ICA in AS treatment.

Main Methods:

  • Atherosclerosis was modeled in ApoE-/- mice and human aortic vascular smooth muscle cells (HAVSMCs).
  • Icariin treatment effects were assessed using lipid accumulation, plaque formation, cell viability, apoptosis, and migration assays.
  • MicroRNA (miRNA) profiling and in silico analyses identified potential signaling pathways, including miR-205-5p.

Main Results:

  • Icariin reduced blood lipid accumulation and plaque formation in AS mice.
  • Icariin promoted apoptosis and inhibited migration in ox-LDL-induced HAVSMCs.
  • The inhibitory effects of ICA on cell proliferation and migration were reversed by silencing miR-205-5p.

Conclusions:

  • Icariin alleviates atherosclerosis by inhibiting proliferation and migration of vascular smooth muscle cells.
  • The therapeutic action of icariin may be mediated by the upregulation of miR-205-5p.
Abstract

Keywords:
AtherosclerosisHuman aortic vascular smooth muscle cellsIcariinmiR-205-5pmiR-205-5p/ERBB4/AKT

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