Oxygen-Rich Graphene/ZnO₂-Ag nanoframeworks with pH-Switchable Catalase/Peroxidase activity as O₂ Nanobubble-Self...

The oxygen-rich organic/inorganic (reduced graphene oxide (rGO)/ZnO₂-Ag) nanoframeworks as suppliers of O₂ nanobubbles (NBs) with dual pH-and-temperature-sensitive behavior were developed to suppress bacterial growth. It was demonstrated that not only the rate but also the final product of oxygen-rich ZnO₂ decomposition (to an intermediate product of H₂O₂) rate was dramatically controlled by pH adjustment. Furthermore, in the presence of Ag nanoparticles, ̇OH radical generation switched to O₂ NBs evolution by shifting the pH from acidic to basic/neutral conditions, demonstrating an adjustable nanozyme function-ability between catalase and peroxidase-like activity, respectively. Antibacterial properties of the in-situ generated O₂ NBs substantially enhanced against bacterial models including methicillin-resistant Staphylococcus aureus in the presence of rGO. In fact, deflecting the electrons from their main respiratory chain to an oxygen-rich bypath through rGO significantly stimulated reactive oxygen species (ROS) generation, combating bacteria more efficiently. Moreover, NIR laser irradiation-induced temperature rise (due to the inherent photothermal properties of rGO) facilitated ZnO₂ decomposition and accelerated growth and collapse of NBs. The simultaneous microscale thermal and mechanical destructions induced stronger antibacterial behavior. These results hold great promises for designing simple organic/inorganic nanoframeworks as solid sources of NBs with tunable enzyme-like ability in response to environmental conditions suitable for forthcoming graphene-based bio-applications.