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Updated: Aug 12, 2025

Quantitative PCR-based Assay to Measure Sonic Hedgehog Signaling in Cellular Model of Ciliogenesis
07:26

Quantitative PCR-based Assay to Measure Sonic Hedgehog Signaling in Cellular Model of Ciliogenesis

Published on: January 31, 2025

643

Downregulation of Sonic hedgehog signaling induces G2-arrest in genital warts

Xiangxi Du1, Shan Li2, Kun Yang1

  • 1Department of Dermatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Skin Research and Technology : Official Journal of International Society for Bioengineering and the Skin (ISBS) [And] International Society for Digital Imaging of Skin (ISDIS) [And] International Society for Skin Imaging (ISSI)
|January 27, 2023
PubMed
Abstract

Insights

Sonic hedgehog (SHh) signaling downregulation promotes human papillomavirus (HPV) replication and genital wart formation by inducing G2/M arrest in keratinocytes. This study investigated the SHh pathway

Area of Science:

  • Dermatology
  • Molecular Biology
  • Virology

Background:

  • Genital warts result from human papillomavirus (HPV) infected keratinocyte dysfunction.
  • The role of Sonic hedgehog (SHh) signaling in genital wart pathogenesis is not well understood.

Purpose of the Study:

  • To investigate the correlation between genital wart formation and SHh signaling.
  • To elucidate the mechanism by which SHh signaling influences HPV-associated keratinocyte changes.

Main Methods:

  • Assessed SHh pathway components (SHh, Ptch1, Gli1) in genital wart tissues and normal foreskin.
  • Detected HPV6/11 using in situ hybridization (ISH).
  • Utilized siRNA to downregulate SHh in HaCaT cells and analyzed cell cycle progression via flow cytometry.

Main Results:

  • SHh, Ptch1, and Gli1 were significantly downregulated in genital warts (condyloma acuminatum, CA) compared to normal tissue.
  • G2-phase cells increased in CA tissues, showing a negative correlation between HPV-DNA and SHh activity.
  • Downregulation of SHh in vitro induced G2 arrest in keratinocytes.

Conclusions:

  • SHh signaling downregulation is implicated in promoting HPV replication and genital wart formation.
  • The mechanism involves inducing G2/M arrest in keratinocytes.
  • Findings suggest SHh pathway as a potential target for therapeutic intervention in HPV-related conditions.
Keywords:
HPV infectionSHh signalingdermatologygenital warts

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