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Updated: Aug 12, 2025

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

Epitope identification for p53R273C mutant.

Jian Zhang^1,2,3, Minglu Liu², Yin Chen³

¹School of Medicine, Nankai University, Tianjin, China.

Immunity, Inflammation and Disease

|January 27, 2023

Summary

This summary is machine-generated.

This study identifies potential neoepitopes from the TP53 R273C mutation, crucial for developing cancer neoantigen immunotherapies. Researchers found specific neoepitopes for HLA-A*02:01 and HLA-A*11:01 alleles.

Keywords:

TP53 R273C mutation immunogenicity immunotherapy neoantigen

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Last Updated: Aug 12, 2025

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Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

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Published on: March 24, 2017

Area of Science:

Oncology
Immunology
Bioinformatics

Background:

Neoantigen identification is critical for cancer immunotherapy development.
The immunogenicity of the TP53 R273C mutation, a common TP53 hotspot, remains unclear.
This study aims to identify potential neoepitopes arising from the p53R273C mutation.

Purpose of the Study:

To identify potential neoepitopes derived from the TP53 R273C mutation.
To assess the immunogenicity of these potential neoepitopes.
To aid in the development of personalized cancer immunotherapies.

Main Methods:

Bioinformatic prediction of peptides with high affinity to common HLA-A alleles (A*11:01, A*02:01).
Peptide synthesis and validation using peptide exchange assays.
Immunogenicity assessment in a human leukocyte antigen (HLA)-A2 transgenic mouse model.

Main Results:

Computational prediction identified peptides with high affinity to HLA-A*11:01 and HLA-A*02:01.
Peptide exchange assays revealed peptides with higher exchange efficiency.
Validation in HLA-A2 transgenic mice confirmed immunogenicity of selected peptides.
Identified three potential neoepitopes for HLA-A*02:01 and one for HLA-A*11:01.

Conclusions:

Three potential neoepitopes for p53^R273C were identified for HLA-A*02:01.
One potential neoepitope for p53^R273C was identified for HLA-A*11:01.
No neoepitopes were identified for HLA-A*24:02, suggesting allele-specific presentation.