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Related Concept Videos

Constitutive and Regulated Gene Expression01:27

Constitutive and Regulated Gene Expression

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Gene expression in prokaryotes is governed by constitutive and regulated systems, allowing cells to balance the production of essential proteins with adaptive responses to environmental changes.Constitutive Gene ExpressionConstitutive, or housekeeping, genes are continuously expressed as they encode proteins vital for fundamental cellular processes. These include enzymes for glycolysis, ribosomal components for protein synthesis, and proteins involved in DNA replication. Their constant...
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What is Gene Expression?01:36

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A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then...
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Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

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Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
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Repressible Operon: trp Operon01:21

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The trp operon in Escherichia coli exemplifies a repressible operon. It regulates the synthesis of tryptophan through repressor-mediated transcriptional control and attenuation. This dual regulatory mechanism ensures tryptophan biosynthesis occurs only when needed, conserving cellular resources.Structure of the trp OperonThe trp operon consists of five structural genes (trpE, trpD, trpC, trpB, and trpA) that encode enzymes for tryptophan biosynthesis. These genes are transcribed as a single...
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Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

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The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
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Gene Regulation During Sporulation01:17

Gene Regulation During Sporulation

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Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...
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Related Experiment Video

Updated: Aug 12, 2025

Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters
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Autorepression-Based Conditional Gene Expression System in Yeast for Variation-Suppressed Control of Protein Dosage.

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  • 1Computational Systems Biology and Swiss Institute of Bioinformatics, ETH Zurich, Basel, Switzerland.

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|January 28, 2023
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Researchers developed a new gene expression control system in yeast that overcomes limitations of existing methods. This system offers tight, precise control and enables synchronized cell cycle progression for advanced research applications.

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Area of Science:

  • Molecular Biology
  • Yeast Genetics
  • Synthetic Biology

Background:

  • Conditional control of gene expression is crucial for studying gene function in Saccharomyces cerevisiae.
  • Existing methods often have drawbacks like leaky expression, specialized equipment, or specific growth conditions.

Purpose of the Study:

  • To develop a novel, robust system for conditional gene expression in yeast.
  • To overcome limitations of current gene control methods, ensuring tight regulation and low variability.
  • To demonstrate a key application in generating synchronized cell populations for cell cycle studies.

Main Methods:

  • Construction of yeast strains using two transformations incorporating a TetR-based repressor system.
  • Implementation of an autorepression loop for TetR to ensure tight control and low expression variation.
  • Utilizing a TetR-Tup1 fusion repressor to eliminate leaky gene expression.
  • Application of the system for cell cycle synchronization by controlling CDC20 expression.

Main Results:

  • Development of yeast strains with a TetR-Tup1 based system for precise, inducible gene expression.
  • Achieved tight control of gene expression with minimal cell-to-cell variation due to autorepression.
  • Demonstrated successful cell cycle synchronization and release by precisely controlling CDC20 expression.
  • The system proved effective for both endogenous and exogenous genes.

Conclusions:

  • The novel TetR-Tup1 system provides a superior method for conditional gene expression in Saccharomyces cerevisiae.
  • This system overcomes significant limitations of previous methods, offering enhanced precision and reproducibility.
  • The ability to synchronize cell populations opens new avenues for studying cell cycle dynamics and gene function.