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Chromatin Landscape Governing Murine Epidermal Differentiation.
Subhashree Nayak1, Kan Jiang2, Emma Hope1
1Laboratory of Skin Biology, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, Maryland, USA.
The Journal of Investigative Dermatology
|January 28, 2023
Summary
This study maps the dynamic chromatin changes during mouse epidermal differentiation. It reveals key regulatory networks and superenhancers controlling gene expression for precise cell development.
Area of Science:
- Cell Biology
- Genomics
- Epigenetics
Background:
- Cell differentiation relies on dynamic chromatin regulation.
- Understanding epidermal differentiation requires mapping its regulatory networks.
Purpose of the Study:
- To define the temporospatial differentiation axis in murine epidermal cells.
- To identify regulatory networks and epigenetic controls governing epidermal differentiation.
Main Methods:
- Generated multi-omics datasets: RNA sequencing, ATAC-seq, Hi-C, and ChIP-seq.
- Analyzed gene expression dynamics, chromatin accessibility, architecture, and histone modifications.
- Investigated basal and suprabasal epidermal cell fractions.
Main Results:
- Identified suppressed genes during differentiation, controlled by superenhancers.
- Revealed the Dlx/Klf/Grhl network's role in epidermal differentiation.
- Characterized differential chromatin compartments, TADs, and looping, especially in the epidermal differentiation complex.
Conclusions:
- Provides a genome-wide resource of chromatin dynamics in epidermal differentiation.
- Uncovers novel regulatory networks and epigenetic control by Dlx3-bound superenhancers.
- Defines the epigenetic landscape of epidermal lineage specification.
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