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Membrane Targeted Azobenzene Drives Optical Modulation of Bacterial Membrane Potential.

Tailise Carolina de Souza-Guerreiro¹, Gaia Bondelli², Iago Grobas³

¹School of Life Sciences, University of Warwick, Coventry, CV4 7AL, UK.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)

|January 29, 2023

Summary

This summary is machine-generated.

Researchers developed a new optical tool, Ziapin2, to control bacterial membrane potential. This azobenzene molecule allows light-induced hyperpolarization, aiding the study of bacterial electrical signaling and physiology.

Keywords:

bacterial cell electrophysiology bacterial electrical signaling bioelectricity nanomaterials optostimulation photonics

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Optical Control of Living Cells Electrical Activity by Conjugated Polymers

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Area of Science:

Microbiology
Biophysics
Molecular Biology

Background:

Bacterial membrane potential is dynamic and crucial for cellular signaling.
Understanding membrane potential regulation is limited by a lack of suitable research tools.
Optical modulation offers a novel approach to study and control bacterial electrical activity.

Purpose of the Study:

To investigate the potential of optical modulation for controlling bacterial membrane potential.
To introduce a membrane-targeted azobenzene, Ziapin2, as a tool for photo-modulating membrane potential in Bacillus subtilis.
To elucidate the mechanisms underlying light-induced membrane potential changes.

Main Methods:

Utilized a membrane-targeted azobenzene (Ziapin2) in Bacillus subtilis.
Exposed bacterial cells to blue-green light (470 nm) to induce Ziapin2 isomerization.
Employed ion-channel-deletion strains and ion channel blockers (IAA-94, KtrAB transporter absence) to investigate the mechanism.

Main Results:

Blue-green light exposure induced hyperpolarization of the bacterial membrane potential.
The hyperpolarization response was significantly attenuated in the presence of the chloride channel blocker IAA-94.
Absence of the KtrAB potassium transporter also reduced the observed hyperpolarization.

Conclusions:

Ziapin2 can be photo-modulated to induce hyperpolarization in bacterial membranes.
Ziapin2-induced hyperpolarization involves the opening of ion channels, specifically influenced by chloride and potassium transport.
Ziapin2 serves as a valuable optical tool for studying bacterial electrical signaling, potentially impacting research in areas like biofilm dynamics and antimicrobial resistance.