Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Chromatin structures: dissecting their mixed patterns in nuclease digests.

R D Drinkwater1, P J Wilson, J D Skinner

  • 1Flinders Medical Centre, Adelaide, SA, Australia.

Nucleic Acids Research
|October 12, 1987
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Decisions, decisions, decisions. A qualitative exploration of decision-making in performance support leaders.

Frontiers in sports and active living·2025
Same author

Developing Decision-Making Expertise in Professional Sports Staff: What We Can Learn from the Good Judgement Project.

Sports medicine - open·2023
Same author

On fragmenting, densely mineralised acellular protrusions into articular cartilage and their possible role in osteoarthritis.

Journal of anatomy·2014
Same author

Cushing's disease: a single centre's experience using the linear accelerator (LINAC) for stereotactic radiosurgery and fractionated stereotactic radiotherapy.

Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia·2013
Same author

Acromegaly: a single centre's experience of stereotactic radiosurgery and radiotherapy for growth hormone secreting pituitary tumours with the linear accelerator.

Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia·2013
Same author

The use of radial symmetry to localize retinal landmarks.

Computerized medical imaging and graphics : the official journal of the Computerized Medical Imaging Society·2013

Chromatin structure analysis reveals distinct DNA digestion patterns. These patterns suggest specific folding models for compact and exposed chromatin, challenging current packing theories.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Understanding chromatin higher-order packing is crucial for gene regulation.
  • Existing coil models may not fully explain chromatin organization.

Purpose of the Study:

  • To analyze DNA digestion patterns in human lymphoblast nuclei using Fe-DNAase-1.
  • To investigate the relationship between DNA digestion patterns and chromatin structure.
  • To evaluate current models of chromatin higher-order packing.

Main Methods:

  • Fe-DNAase-1 digestion of human lymphoblast nuclei.
  • Analysis of DNA repeat patterns (di-nucleosomal (2N) and mono-nucleosomal (1N)).
  • Hybridization studies of DNA sequences.

Related Experiment Videos

Main Results:

  • Identified distinct digestion features: 2N repeat, 1N repeat, random DNA, and triple peak splitting.
  • The random DNA component aligns with the face-to-face layering model.
  • 2N patterns correlate with compact chromatin, 1N with exposed chromatin.
  • Repetitive and transcriptionally active DNA share similar digestion spectra.

Conclusions:

  • Proposed models of nucleofilament folding (back-to-back and face-to-face) explain observed patterns.
  • Current coil models are insufficient for explaining higher-order chromatin packing.