Related Experiment Videos
Selective catalysis of A.T base pair proton exchange in DNA complexes: imino proton NMR analysis
L Strekowski1, R A Watson, W D Wilson
1Department of Chemistry, Georgia State University, Atlanta 30303.
Nucleic Acids Research
|October 26, 1987
Abstract:
Polyaromatic molecules with amino chain substituents, upon binding with DNA, selectively catalyze exchange of the A.T base pair protons with bulk water protons. The amine-catalyzed exchange is mediated by compounds which are A.T and G.C base sequence specific, intercalators, and outside binders. A mechanism for the selective exchange, involving transient opening and closing of individual A.T base pairs in the duplex, is discussed.