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Transcription01:10

Transcription

147.5K
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
147.5K
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

969
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
969
Responses to Heat and Cold Stress02:45

Responses to Heat and Cold Stress

13.7K
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
13.7K
Photoreceptors and Plant Responses to Light02:00

Photoreceptors and Plant Responses to Light

20.6K
Light plays a significant role in regulating the growth and development of plants. In addition to providing energy for photosynthesis, light provides other important cues to regulate a range of developmental and physiological responses in plants.
20.6K
Translational Regulation01:29

Translational Regulation

66
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
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Cell Signaling in Plants01:25

Cell Signaling in Plants

5.7K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.7K

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Related Experiment Video

Updated: Aug 12, 2025

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis
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Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis

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Light and temperature regulate m6A-RNA modification to regulate growth in plants.

Oliver Artz1, Amanda Ackermann1, Laura Taylor1

  • 1Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, 11724. USA.

Biorxiv : the Preprint Server for Biology
|January 30, 2023
PubMed
Summary

N6-methyladenosine (m6A) modification in plants is explored for its role in gene expression under varying light and temperature. This study reveals m6A influences RNA localization and alternative polyadenylation, fine-tuning gene regulation.

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Area of Science:

  • Plant molecular biology
  • Epigenetics
  • Transcriptomics

Background:

  • N6-methyladenosine (m6A) is a crucial mRNA modification regulating gene expression.
  • Plants utilize complex gene regulatory networks to adapt to environmental changes like light and temperature.
  • The function of m6A in plant environmental response remains largely uninvestigated.

Approach:

  • Transcriptome-wide m6A mapping was performed under diverse light and temperature conditions.
  • Analysis focused on the spatial distribution of m6A modifications on plant transcripts.
  • Investigated the correlation between m6A, transcript localization, and alternative polyadenylation.

Key Points:

  • m6A modifications exhibit specific distributions, enriched in 5'UTR and near transcriptional end sites.
  • m6A presence is linked to alternative polyadenylation, favoring shorter 3'UTRs.
  • Shorter 3'UTRs influenced by m6A may regulate transcript abundance and localization.

Conclusions:

  • Environmental stimuli can alter the plant m6A landscape.
  • m6A-driven changes in alternative polyadenylation and transcript localization fine-tune gene expression in response to environmental cues.
  • This study provides novel insights into epigenetic regulation in plants under variable conditions.