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Related Concept Videos

Proteomics01:33

Proteomics

7.6K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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Related Experiment Video

Updated: Aug 12, 2025

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling
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Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling

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MASH Native: A Unified Solution for Native Top-Down Proteomics Data Processing.

Eli J Larson, Melissa R Pergande, Michelle E Moss

    Biorxiv : the Preprint Server for Biology
    |January 30, 2023
    PubMed
    Summary
    This summary is machine-generated.

    MASH Native offers a unified software solution for native top-down proteomics (nTDP) data analysis. This user-friendly tool aids in characterizing protein complexes and proteoforms, integrating native mass spectrometry and top-down proteomics.

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    Related Experiment Videos

    Last Updated: Aug 12, 2025

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    Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
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    Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

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    Area of Science:

    • Proteomics
    • Biochemistry
    • Computational Biology

    Background:

    • Native top-down proteomics (nTDP) combines native mass spectrometry (nMS) and top-down proteomics (TDP) for comprehensive protein analysis.
    • Existing software lacks a unified, user-friendly platform for nTDP data processing.

    Approach:

    • Developed MASH Native, a unified software package for nTDP data analysis.
    • MASH Native features a user-friendly interface with database searching capabilities.
    • Supports diverse data formats and includes options for deconvolution, database searching, and spectral summing.

    Key Points:

    • Enables simultaneous characterization of protein complexes and proteoforms.
    • Provides a one-stop solution for complex nTDP datasets.
    • Facilitates proteoform identification and characterization.

    Conclusions:

    • MASH Native addresses the need for integrated nTDP data analysis software.
    • The software enhances the comprehensive analysis of protein complexes and proteoforms.
    • Freely available with tutorials and documentation to support users.