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Related Concept Videos

Protein Networks02:26

Protein Networks

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An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
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Label-Free Quantitative Proteomics Workflow for Discovery-Driven Host-Pathogen Interactions
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Next-generation interaction proteomics for quantitative Jumbophage-bacteria interaction mapping.

Andrea Fossati, Deepto Mozumdar, Claire Kokontis

    Biorxiv : the Preprint Server for Biology
    |January 30, 2023
    PubMed
    Summary
    This summary is machine-generated.

    This study introduces co-fractionation mass spectrometry (SEC-MS) to map host-pathogen interactions (HPIs) in jumbophage infections. The method reveals thousands of interactions, uncovering conserved predation mechanisms and novel phage components.

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    Area of Science:

    • Microbiology
    • Virology
    • Biochemistry

    Background:

    • Host-pathogen interactions (HPIs) are crucial for infection dynamics.
    • Traditional methods for HPIs analysis are limited to small pathogens due to targeted expression of viral proteins.
    • High-throughput analysis of HPIs in complex infections is needed.

    Approach:

    • Co-fractionation mass spectrometry (SEC-MS) was employed for high-throughput HPIs analysis.
    • Studied native infections of jumbophages (ϕKZ and ϕPA3) in Pseudomonas aeruginosa.
    • Developed PhageMAP, an online resource for interactome data visualization and prediction.

    Key Points:

    • Detected over 6000 host-pathogen and 200 pathogen-pathogen interactions per phage, covering >50% of the phage proteome.
    • Identified conserved HPIs mechanisms within the KZ-like phage family.
    • Discovered 23 novel virion components, 5 novel injected proteins, and interactions with the host ribosome.

    Conclusions:

    • SEC-MS enables scalable profiling of HPIs and protein complex dynamics during infection.
    • Revealed conserved phage predation mechanisms and identified novel phage components.
    • The PhageMAP resource facilitates further research into phage biology and HPIs.