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Self-Assembly of Microtubule Tactoids
Published on: June 23, 2022
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CTCF is essential for proper mitotic spindle structure and anaphase segregation.
Katherine Chiu1, Yasmin Berrada1, Nebiyat Eskndir1
1Biology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Biorxiv : the Preprint Server for Biology
|January 30, 2023
Summary
CTCF knockdown disrupts mitosis, causing prolonged cell division and failed genome segregation. This impacts nuclear size and shape, highlighting CTCF's role in mitotic fidelity.
Area of Science:
- Cell Biology
- Genetics
Background:
- Mitosis ensures equal genome distribution to daughter cells.
- The role of CTCF (CCCTC-binding factor) in mitotic fidelity is not well understood.
Approach:
- Investigated CTCF's function in mitosis using CRISPR-based knockdowns in wild-type cells.
- Utilized time-lapse and immunofluorescence imaging to analyze mitotic behaviors and spindle organization.
- Assessed cell cycling and mitotic checkpoint activation.
Key Points:
- CTCF knockdown leads to prolonged mitosis and failed anaphase segregation.
- Disorganization of the mitotic spindle, including tri/tetrapolar spindles, was observed.
- Nuclear size increased in interphase following CTCF knockdown, indicating genome segregation failure.
Conclusions:
- CTCF is crucial for maintaining mitotic fidelity, ensuring proper metaphase organization and anaphase segregation.
- Disruption of CTCF function affects the cell cycle and interphase nuclear morphology.
- Individual mitotic behaviors are critical for accurate genome division, which population-level analysis may obscure.
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