Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Simple CsI doping outperforms complex organic additives in carbon-based perovskite solar cells.

Nanoscale advances·2026
Same author

Risk Factors for Postoperative Growth Retardation in Children with Biliary Atresia After Kasai Portoenterostomy: A Retrospective Analysis.

Journal of pediatric surgery·2026
Same author

Impaired sleep resilience underlies transient neural instability in insomnia disorder.

iScience·2026
Same author

Plaque characteristics and clinical outcomes of non-culprit long lesions in patients with acute myocardial infarction.

International journal of cardiology·2026
Same author

Management of congenital duodenal obstruction in neonates: a 10-year retrospective cohort study from a National Children's Hospital.

Surgical endoscopy·2026
Same author

Intraoperative quantitative angiography predicts midterm left subclavian artery patency after thoracic endovascular aortic repair.

Quantitative imaging in medicine and surgery·2026

Related Experiment Video

Updated: Aug 12, 2025

A Mouse Model of Chronic Liver Fibrosis for the Study of Biliary Atresia
09:12

A Mouse Model of Chronic Liver Fibrosis for the Study of Biliary Atresia

Published on: February 3, 2023

2.6K

Identifying and validating molecular subtypes of biliary atresia using multiple high-throughput data integration

Dingding Wang1, Shen Yang1, Yong Zhao1

  • 1Department of Neonatal Surgery, Beijing Children's Hospital, Capital Medical University, Beijing, China.

Frontiers in Immunology
|January 30, 2023
PubMed
Summary

This study identified four molecular subtypes of biliary atresia (BA) based on gene expression. These subtypes, including autoimmune, inflammatory, viral, and oxidative stress, show distinct prognoses, paving the way for personalized BA treatment strategies.

Keywords:
biliary atresiabiliary fibrosisimmunemolecular subtypesprognosis

More Related Videos

Comparative Lesions Analysis Through a Targeted Sequencing Approach
08:16

Comparative Lesions Analysis Through a Targeted Sequencing Approach

Published on: November 5, 2019

6.8K
Generation and Quantitative Characterization of Functional and Polarized Biliary Epithelial Cysts
09:55

Generation and Quantitative Characterization of Functional and Polarized Biliary Epithelial Cysts

Published on: May 16, 2020

3.9K

Related Experiment Videos

Last Updated: Aug 12, 2025

A Mouse Model of Chronic Liver Fibrosis for the Study of Biliary Atresia
09:12

A Mouse Model of Chronic Liver Fibrosis for the Study of Biliary Atresia

Published on: February 3, 2023

2.6K
Comparative Lesions Analysis Through a Targeted Sequencing Approach
08:16

Comparative Lesions Analysis Through a Targeted Sequencing Approach

Published on: November 5, 2019

6.8K
Generation and Quantitative Characterization of Functional and Polarized Biliary Epithelial Cysts
09:55

Generation and Quantitative Characterization of Functional and Polarized Biliary Epithelial Cysts

Published on: May 16, 2020

3.9K

Area of Science:

  • Hepatology
  • Molecular Biology
  • Genomics

Background:

  • Biliary atresia (BA) is a leading cause of neonatal obstructive jaundice with multifactorial etiology.
  • Current treatment strategies for BA are uniform despite heterogeneous pathological features and outcomes.
  • Identifying molecular subtypes is crucial for developing personalized treatment approaches.

Purpose of the Study:

  • To perform integrative clustering analysis on high-throughput datasets to identify molecular subtypes of BA.
  • To establish a basis for a new, personalized treatment strategy for BA patients based on identified subtypes.

Main Methods:

  • Downloaded and analyzed RNA sequence data (GSE122340) from the GEO database.
  • Collected and performed transcriptome sequencing on 31 BA and 20 control liver tissues.
  • Utilized integrated unsupervised cluster analysis incorporating gene expression, fibrosis, and immune scores, validated on independent datasets.

Main Results:

  • Identified four distinct molecular subtypes of BA: autoimmune, inflammatory, virus infection-related, and oxidative stress.
  • Each subtype exhibited unique biological processes and prognoses, with the inflammatory subtype showing the best prognosis and the virus infection-related subtype the worst.
  • Results were independently validated, confirming the distinct characteristics and prognostic implications of each subtype.

Conclusions:

  • Four molecular subtypes of BA with distinct prognoses and biological processes were identified.
  • Individualized perioperative and preoperative treatment strategies tailored to BA subtypes may improve patient outcomes.
  • This classification offers a novel approach to personalized medicine for biliary atresia.