Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

5 alpha-reductase activity in rat adipose tissue.

M Zyirek1, C Flood, C Longcope

  • 1Department of Obstetrics/Gynecology, University of Massachusetts Medical School, Worcester 01605.

Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)
|November 1, 1987
PubMed
Summary

Rat adipose tissue contains 5 alpha-reductase activity, primarily in stromal cells. Estradiol significantly reduces this activity, suggesting hormonal regulation of steroid metabolism in fat tissue.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

"Loneliness can also kill:" a qualitative exploration of outcomes and experiences of the SUPERB peer-befriending scheme for people with aphasia and their significant others.

Disability and rehabilitation·2021
Same author

"For them and for me": a qualitative exploration of peer befrienders' experiences supporting people with aphasia in the SUPERB feasibility trial.

Disability and rehabilitation·2021
Same author

Midazolam use for dental conscious sedation: how safe are we?

British dental journal·2018
Same author

Severe mental illness and type 2 diabetes: using theory and research evidence to develop complex behaviour change interventions.

Journal of psychiatric and mental health nursing·2016
Same author

Is knowledge and practice safer in England after the release of national guidance on the resuscitation of patients in mental health and learning disabilities?

Journal of psychiatric and mental health nursing·2013
Same author

A report from #BlueJC: can antenatal hypnosis reduce the use of pharmacological analgesia in labour?

BJOG : an international journal of obstetrics and gynaecology·2013

Area of Science:

  • Endocrinology
  • Steroid Metabolism
  • Adipose Tissue Biology

Background:

  • 5 alpha-reductase is a key enzyme in androgen metabolism.
  • Adipose tissue is increasingly recognized for its endocrine functions.
  • Understanding steroid metabolism in adipocytes and stromal cells is crucial.

Purpose of the Study:

  • To quantify 5 alpha-reductase activity in different rat adipose tissue depots.
  • To compare activity between adipocytes and stromal cells.
  • To investigate the effect of estradiol on 5 alpha-reductase activity.

Main Methods:

  • Isolation of adipocytes and stromal cells from rat epididymal, perinephric, and subcutaneous fat pads.
  • Measurement of 5 alpha-reductase activity via [3H]dihydrotestosterone formation from [3H]testosterone.

Related Experiment Videos

  • Incubation with estradiol (10(-8) M) to assess hormonal effects.

    • Main Results:

    • Stromal cells exhibited higher 5 alpha-reductase activity than adipocytes across depots.
    • Epididymal fat pad stromal cells showed the highest activity compared to other depots.
    • Estradiol treatment resulted in a 90% reduction in 5 alpha-reductase activity.
    • Aromatase activity was negligible in all studied tissues.

    Conclusions:

    • Rat adipose stromal cells possess significant 5 alpha-reductase activity, with depot-specific variations.
    • Estradiol plays a potent inhibitory role in regulating 5 alpha-reductase activity in adipose tissue.
    • These findings highlight the role of adipose tissue in local steroid metabolism and its modulation by hormones.