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Multilayer Heterogeneous Membrane Biosensor Based on Multiphysical Field Coupling for Human Serum Albumin Detection.
Haoyu Wang1, Pengli Xiao1, Shengbo Sang1
1Shanxi Key Laboratory of Micro Nano Sensors & Artificial Intelligence Perception, College of Information and Computer, Taiyuan University of Technology, Taiyuan 030024, China.
ACS Omega
|January 30, 2023
Summary
This study introduces a novel multilayer biosensor for highly sensitive detection of human serum albumin (HSA), a key indicator of kidney disease. The developed biosensor shows potential for early disease screening by detecting HSA at low concentrations.
Area of Science:
- Biomedical Engineering
- Materials Science
- Clinical Diagnostics
Background:
- Abnormal human serum albumin (HSA) levels in bodily fluids are linked to renal disease status.
- Surface stress biosensors offer potential for protein detection but require enhanced sensitivity.
- Existing biosensors need improvement for accurate and early detection of disease biomarkers.
Purpose of the Study:
- To develop a highly sensitive multilayer heterogeneous membrane biosensor (MHBios) for human serum albumin (HSA) detection.
- To enhance biosensor sensitivity through magnetic sensitization and coupling of multiple fields.
- To validate the biosensor's performance for early disease screening using actual samples.
Main Methods:
- Fabrication of a MXene/PDMS/Fe3O4/PDMS multilayer heterogeneous membrane biosensor (MHBios).
- Utilized magnetic sensitization by applying a uniform magnetic field to amplify signal transduction.
- Antibody modification for specific HSA binding and conversion of surface stress to electrical signals.
Main Results:
- Achieved a low detection limit (LOD) of 78 ng/mL for HSA detection within a 0-50 μg/mL range.
- Demonstrated significantly lower LOD compared to the minimum diagnostic limit for microalbuminuria.
- Successfully detected HSA in actual patient samples, validating real-world applicability.
Conclusions:
- The developed MHBios exhibits high sensitivity and specificity for HSA detection.
- Magnetic sensitization effectively enhances the biosensor's signal transduction capabilities.
- The MHBios holds significant potential for early diagnosis and screening of renal diseases.
