Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Suture residues are associated with histomorphological changes in failed anterior cruciate ligament hamstring autografts.

Journal of experimental orthopaedics·2026
Same author

[Perioperative Molecular Testing in Non-small Cell Lung Cancer].

Zentralblatt fur Chirurgie·2026
Same author

Pitfalls in Detecting MET Exon 14 Skipping Variants by DNA- and RNA-Based Next-Generation Sequencing Technologies in a Large Real-World Cohort and Results of the First Multinational External Quality Assessment Schemes.

The Journal of molecular diagnostics : JMD·2026
Same author

Clinical impact of molecular tumor board inclusion on treatment outcomes in neuroendocrine neoplasms.

Neuroendocrinology·2026
Same author

An agentic framework for autonomous scientific discovery in cancer pathology.

Nature medicine·2026
Same author

The genetic landscape of extramedullary plasmacytoma: a comparative analysis with extramedullary disease of multiple myeloma.

Haematologica·2026

Related Experiment Video

Updated: Aug 12, 2025

A Fish-feeding Laboratory Bioassay to Assess the Antipredatory Activity of Secondary Metabolites from the Tissues of Marine Organisms
16:03

A Fish-feeding Laboratory Bioassay to Assess the Antipredatory Activity of Secondary Metabolites from the Tissues of Marine Organisms

Published on: January 11, 2015

9.5K

MET-FISH Evaluation Algorithm: Proposal of a Simplified Method.

Roberta Castiglione1,2, Christina Alidousty2, Barbara Holz2

  • 1Institute of Pathology, Klinikum Stuttgart, Stuttgart, Germany.

Journal of Cancer Science and Clinical Therapeutics
|January 30, 2023
PubMed
Summary

A faster diagnostic algorithm for MET amplifications (METamp) in non-small cell lung cancer (NSCLC) was developed. Evaluating 20 cells using Fluorescence In-Situ Hybridization (FISH) provides reliable results, reducing analysis time.

Keywords:
Evaluation AlgorithmFishMET-AmplificationNon-Small-Cell-Lung Cancer

More Related Videos

Using the FishSim Animation Toolchain to Investigate Fish Behavior: A Case Study on Mate-Choice Copying In Sailfin Mollies
10:50

Using the FishSim Animation Toolchain to Investigate Fish Behavior: A Case Study on Mate-Choice Copying In Sailfin Mollies

Published on: November 8, 2018

10.9K
DNA-based Fish Species Identification Protocol
09:15

DNA-based Fish Species Identification Protocol

Published on: April 28, 2010

29.6K

Related Experiment Videos

Last Updated: Aug 12, 2025

A Fish-feeding Laboratory Bioassay to Assess the Antipredatory Activity of Secondary Metabolites from the Tissues of Marine Organisms
16:03

A Fish-feeding Laboratory Bioassay to Assess the Antipredatory Activity of Secondary Metabolites from the Tissues of Marine Organisms

Published on: January 11, 2015

9.5K
Using the FishSim Animation Toolchain to Investigate Fish Behavior: A Case Study on Mate-Choice Copying In Sailfin Mollies
10:50

Using the FishSim Animation Toolchain to Investigate Fish Behavior: A Case Study on Mate-Choice Copying In Sailfin Mollies

Published on: November 8, 2018

10.9K
DNA-based Fish Species Identification Protocol
09:15

DNA-based Fish Species Identification Protocol

Published on: April 28, 2010

29.6K

Area of Science:

  • Oncology
  • Molecular Diagnostics
  • Genetics

Background:

  • MET amplifications (METamp) are implicated in 5% of non-small cell lung cancer (NSCLC) cases.
  • METamp often signifies resistance to ALK and/or EGFR-targeted therapies.
  • Fluorescence In-Situ Hybridization (FISH) is the gold-standard for METamp detection, particularly for subclonality, but current evaluation algorithms are time-consuming.

Purpose of the Study:

  • To develop a faster and equally reliable diagnostic algorithm for METamp detection.
  • To streamline the classification of METamp into negativity, low, intermediate, and high-level amplification categories.

Main Methods:

  • Retrospective analysis of MET-FISH data from 497 NSCLC cases.
  • Comparison of diagnostic results obtained by evaluating 20 cells versus the standard 60 cells.
  • Development of a simplified FISH algorithm involving initial screening, evaluation of 20 cells, and optional further counting for equivocal cases.

Main Results:

  • Evaluating 20 cells yielded identical results to the 60-cell method in 93.4% (464/497) of cases.
  • Discrepancies occurred in 5.6% (33/497) of cases, leading to incorrect classification (25 upgrades, 8 downgrades).
  • The proposed simplified algorithm defines clear criteria for immediate sign-out (negative or high-level amplification) and an 'equivocal' category requiring further analysis.

Conclusions:

  • A simplified MET FISH algorithm evaluating 20 cells is a reliable and faster alternative to the standard 60-cell count.
  • This approach significantly reduces diagnostic time while maintaining accuracy for METamp detection in NSCLC.
  • The defined 'equivocal' category ensures thoroughness for cases requiring additional investigation, optimizing diagnostic efficiency.