Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Decoding the Gut-Liver Crosstalk: Microbial Metabolite- Driven AhR Signalling Networks in MASLD Pathogenesis.

International immunopharmacology·2026

Same author

Development of a predictive model for depressive symptoms in type 2 diabetes mellitus patients under community management: Based on visual function index.

Ibrain·2026

Same author

Comment on "Prevalence of Burning Mouth Syndrome in Parkinson's Disease: A Prospective Case-Control Study".

Oral diseases·2026

Same author

Recruitability during General Anesthesia: Comment.

Anesthesiology·2026

Same author

Ononin combined with <i>Lactobacillus paracasei</i> alleviates DSS-induced ulcerative colitis by ferroptosis via inhibiting JAK2/STAT3 signaling pathway.

Biochemistry and biophysics reports·2026

Same author

Comparing Minimally Invasive Modified Levator vs. Conjoint Fascia Sheath-Levator Suspension in Correcting Severe Congenital Ptosis.

Aesthetic plastic surgery·2026

Search research articles

Related Experiment Video

Updated: Aug 12, 2025

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Published on: January 3, 2025

High-resolution quantitative trait locus mapping for rice grain quality traits using genotyping by sequencing.

Su-Kui Jin^1,2,3,4,5, Li-Na Xu⁵, Qing-Qing Yang^1,2,3,4

¹JiangsuKey Laboratory of Crop Genomics and Molecular Breeding, College of Agriculture, Yangzhou University, Yangzhou, China.

Frontiers in Plant Science

|January 30, 2023

Summary

Researchers identified novel quantitative trait loci (QTLs) for rice grain quality using high-density genetic mapping. These findings will accelerate breeding for improved rice varieties and enhance global food security.

Keywords:

genotyping by sequencing grain quality high-density genetic map quantitative trait locus rice

More Related Videos

Identifying Mutations by High Resolution Melting in a TILLING Population of Rice

Identifying Mutations by High Resolution Melting in a TILLING Population of Rice

Published on: September 2, 2019

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling

Published on: May 21, 2020

Related Experiment Videos

Last Updated: Aug 12, 2025

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Published on: January 3, 2025

Identifying Mutations by High Resolution Melting in a TILLING Population of Rice

Identifying Mutations by High Resolution Melting in a TILLING Population of Rice

Published on: September 2, 2019

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling

Published on: May 21, 2020

Area of Science:

Plant genetics and breeding
Agricultural science
Molecular biology

Background:

Rice is a staple food for over half the world's population, with increasing demand for high-quality grains.
Improving rice grain quality is crucial for meeting global food needs and enhancing nutritional value.

Purpose of the Study:

To conduct high-resolution quantitative trait loci (QTL) mapping for key rice grain quality traits.
To identify novel genetic loci controlling traits such as whole grain percentage, head rice percentage, and chalkiness.

Main Methods:

Utilized a genotyping-by-sequencing approach to construct a high-density genetic map from an F2 population.
Mapped 3,830 single nucleotide polymorphism markers across 12 linkage groups.
Investigated seven grain quality traits in the F2 population.

Main Results:

Identified 15 QTLs with logarithm of the odds (LOD) scores >4 on chromosomes 6, 7, and 9.
These QTLs explained 0.01%-61.64% of the phenotypic variation for traits including transparency and chalkiness.
Discovered that 14 out of 15 identified QTLs were novel, with only one overlapping previously reported loci.

Conclusions:

The identified novel QTLs provide valuable genetic resources for rice quality improvement.
These findings will facilitate fine mapping and QTL pyramiding for enhanced rice breeding programs.
Accelerated genetic improvement of rice grain quality is expected, contributing to global food security.