Simultaneous Quantitative Analysis of Salmeterol and Fluticasone in Inhalation Spray Using HPLC and a Fast Spectrophotometric Technique Combined with a Time Series Neural Network and Multivariate Calibration Methods
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Maryam Valizadeh1, Zahra Ameri Braki1, Erfan Smiley1
1Islamic Azad University, Department of Chemistry, North Tehran Branch, Vafadar Blvd., Shahid Sadoughi St., Hakimiyeh Exit, Shahid Babaee Highway, Tehran 1651153311, Iran.
Background:
Chromatographic methods have been used for the simultaneous determination of salmeterol (SMT) and fluticasone (FLU). The methods take a lot of analysis time, need sample pre-treatment and large amounts of solvents, and are costly.
Objective:
The aim of this paper was to propose a simple, quick, and low-cost method for the determination of SMT and FLU using a time series neural network and multivariate calibration methods, including partial least-squares (PLS) and principal component regression (PCR).
Methods:
The simultaneous spectrophotometric determination of SMT and FLU in binary mixtures and anti-asthma spray was performed by applying multivariate calibration methods and an intelligent approach.
Results:
The coefficient of determination (R2) of the time series neural network was obtained: 1 and 0.9997 for SMT and FLU, respectively. The mean recovery of PLS was found to be 99.29% and 99.84% for SMT and FLU, respectively. Also, the mean recovery related to the PCR method was 102.05% and 103.72% for SMT and FLU, respectively.
Conclusion:
The inhalation spray was assessed using HPLC and the results were compared with chemometrics methods via an analysis of variance (ANOVA) test.
Highlights:
Intelligent and multivariate calibration methods were proposed. Simultaneous spectrophotometric determination of salmeterol and fluticasone was studied in an anti-asthma spray. HPLC as a reference method was performed and compared with chemometrics methods. Rapid, simple, low-cost, and accurate are the benefits of the proposed approaches.
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