Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cytoskeletal Coordination in Cell Migration01:32

Cytoskeletal Coordination in Cell Migration

4.8K
A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker...
4.8K
Cell Migration01:19

Cell Migration

5.0K
Cell migration is a process by which the cells move from one location to another, playing an essential role in embryological development, repair and regeneration, immune response, and metastasis. Cells migrate in response to chemical or mechanical signals generated by specific organs or tissues. The overall mechanism includes three steps - polarization, protrusion, and release. Polarization involves the formation of a distinct cell front and rear, which determines the direction of movement.
5.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A deficiency screen of the X chromosome for Rap1 GTPase dominant interacting genes in Drosophila border cell migration.

G3 (Bethesda, Md.)·2025
Same author

Expect the unexpected: conventional and unconventional roles for cadherins in collective cell migration.

Biochemical Society transactions·2023
Same author

A Drosophila RNAi screen reveals conserved glioblastoma-related adhesion genes that regulate collective cell migration.

G3 (Bethesda, Md.)·2021
Same author

Sculpting new structures.

eLife·2020
Same author

Protein phosphatase 1 activity controls a balance between collective and single cell modes of migration.

eLife·2020
Same author

Identifying conserved molecular targets required for cell migration of glioblastoma cancer stem cells.

Cell death & disease·2020

Related Experiment Video

Updated: Aug 12, 2025

Quantitative Analysis of Random Migration of Cells Using Time-lapse Video Microscopy
07:27

Quantitative Analysis of Random Migration of Cells Using Time-lapse Video Microscopy

Published on: May 13, 2012

16.9K

Quantitative Image Analysis of Dynamic Cell Behaviors During Border Cell Migration.

Yujun Chen1, Nirupama Kotian1, Jocelyn A McDonald2

  • 1Division of Biology, Kansas State University, Manhattan, KS, USA.

Methods in Molecular Biology (Clifton, N.J.)
|January 30, 2023
PubMed
Summary

This study details methods for observing collective cell migration in Drosophila border cells using live imaging. These protocols enable detailed analysis of cell behaviors and protein dynamics in developing tissues.

Keywords:
Border cellCell protrusionsCell shapeCollective cell migrationConfocal microscopyEgg chamberEx vivo culturingFluorescent microscopyLive imagingProtein dynamics

More Related Videos

Imaging Neutrophil Migration in the Mouse Skin to Investigate Subcellular Membrane Remodeling Under Physiological Conditions
08:41

Imaging Neutrophil Migration in the Mouse Skin to Investigate Subcellular Membrane Remodeling Under Physiological Conditions

Published on: May 10, 2022

1.6K
In vitro Cell Migration and Invasion Assays
09:55

In vitro Cell Migration and Invasion Assays

Published on: June 1, 2014

138.7K

Related Experiment Videos

Last Updated: Aug 12, 2025

Quantitative Analysis of Random Migration of Cells Using Time-lapse Video Microscopy
07:27

Quantitative Analysis of Random Migration of Cells Using Time-lapse Video Microscopy

Published on: May 13, 2012

16.9K
Imaging Neutrophil Migration in the Mouse Skin to Investigate Subcellular Membrane Remodeling Under Physiological Conditions
08:41

Imaging Neutrophil Migration in the Mouse Skin to Investigate Subcellular Membrane Remodeling Under Physiological Conditions

Published on: May 10, 2022

1.6K
In vitro Cell Migration and Invasion Assays
09:55

In vitro Cell Migration and Invasion Assays

Published on: June 1, 2014

138.7K

Area of Science:

  • Developmental biology
  • Cell biology
  • Genetics

Background:

  • Drosophila border cells are a model for collective cell migration.
  • Similarities exist with other migrating cell collectives, such as protrusion formation and actomyosin contractility.
  • Understanding these dynamics is crucial for developmental processes.

Purpose of the Study:

  • To describe protocols for ex vivo culture and live imaging of Drosophila border cells.
  • To enable detailed observation of dynamic cellular behaviors during migration.
  • To provide methods for quantifying cell behaviors and protein dynamics.

Main Methods:

  • Preparation of ex vivo stage 9 Drosophila egg chambers.
  • Live time-lapse imaging using widefield epifluorescent or confocal microscopy.
  • Quantification of cellular behaviors and protein dynamics using Fiji/ImageJ.

Main Results:

  • Established protocols for live imaging of border cell migration.
  • Demonstrated methods for analyzing dynamic cell behaviors.
  • Provided tools for quantifying protein dynamics in migrating cells.

Conclusions:

  • The described methods facilitate the study of collective cell migration in Drosophila.
  • These protocols can be adapted for other migrating cell collectives in cultured tissues.
  • This work provides a foundation for further research into cell migration mechanisms.