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Microfluidics in Assessing Platelet Function
Published on: November 8, 2024
995
Evaluation of survival and functionality in frozen platelets.
Jhon Alexander Avila Rueda1, Jose Acosta2, Oscar Rabinovich2
1Hospital Británico de Buenos Aires; Buenos Aires, Argentina; Hospital Privado Santa Clara De Asís, Salta, Argentina.
Hematology, Transfusion and Cell Therapy
|January 30, 2023
Summary
Cryopreservation effectively extends platelet survival and function for up to 28 days. Different cryopreservative solutions impact platelet viability and aggregation, with specific formulations showing superior results for transfusion readiness.
Area of Science:
- Hematology
- Transfusion Medicine
- Biopreservation
Background:
- Platelets are crucial for hemostasis but have a limited shelf-life.
- Cryopreservation offers a potential method to extend platelet viability and availability.
Purpose of the Study:
- To evaluate the efficacy of cryopreservation in prolonging platelet survival and functionality for up to 28 days.
- To compare the impact of different cryopreservative solutions on post-thaw platelet quality.
Main Methods:
- Apheresis platelets were cryopreserved using solutions containing DMSO with albumin, NaCl, or dextrose.
- Platelets were thawed at 7, 14, and 28 days post-cryopreservation.
- In vitro survival, viability (CD41, CD61, CD42a expression), and functionality (aggregation response) were assessed.
Main Results:
- Cryopreserved platelets maintained viability above 50% for up to 28 days.
- Platelet count and membrane antigen expression varied among cryopreservative groups.
- Platelet aggregation response was best preserved in the group using DMSO5%+NaCl0,9%.
Conclusions:
- Cryopreservation is a viable method for extending platelet shelf-life to 28 days.
- Cryopreservative solution composition significantly influences post-thaw platelet quality and function.
- Further optimization of cryopreservation protocols can enhance the utility of stored platelets.
