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Updated: Aug 12, 2025

Analysis of Histone Antibody Specificity with Peptide Microarrays
Published on: August 1, 2017
Characterization of a polyclonal antibody that is highly selective for the D-isoAsp-25 variant of mammalian histone
Dana W Aswad1, Kevin S O'Leary2, Katherine Williams2
1Department of Molecular Biology and Biochemistry, University of California, Irvine, CA, 92697-3900, USA. dwaswad@uci.edu.
Abstract:
Approximately 12% of histone H2B molecules in mammalian brain contain a modification wherein Asp25 is present as the D-enantiomer, and is mostly linked to Gly26 via the side-chain carboxyl. Here we (1) demonstrate the high specificity of a polyclonal antibody to this modification, and (2) use this Ab to demonstrate that this modification is enriched in brain relative to liver, thymus, and HeLa cells.
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