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$Microcrystallography of Protein Crystals and In Cellulo Diffraction$

Microcrystallography of Protein Crystals and In Cellulo Diffraction

Published on: July 21, 2017

High-throughput phase elucidation of polycrystalline materials using serial rotation electron diffraction.

Yi Luo^1,2, Bin Wang¹, Stef Smeets³

¹Department of Materials and Environmental Chemistry, Stockholm University, Stockholm, Sweden.

Nature Chemistry

|January 30, 2023

Summary

This summary is machine-generated.

Serial rotation electron diffraction (SerialRED) rapidly identifies multiple zeolite phases in complex mixtures. This technique overcomes limitations of X-ray diffraction for ultra-low content and morphologically similar crystalline materials.

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Area of Science:

Materials Science
Crystallography
Chemistry

Background:

Rapid phase elucidation of polycrystalline materials is crucial for new chemical, pharmaceutical, and industrial applications.
Current X-ray diffraction methods face limitations with small crystal sizes and quantities, creating bottlenecks in materials development and high-throughput synthesis screening.

Purpose of the Study:

To demonstrate the application of serial rotation electron diffraction (SerialRED) for high-throughput phase identification of complex polycrystalline zeolite products.
To showcase SerialRED's capability in analyzing materials synthesized with multiple framework T atoms and simple organic structure-directing agents.

Main Methods:

Utilized serial rotation electron diffraction (SerialRED) for analyzing polycrystalline zeolite products.
Investigated materials prepared with diverse framework T atoms ([Si,Ge,Al] or [Si,Ge,B]) and a single organic structure-directing agent.

Main Results:

Successfully identified five distinct zeolite phases from a highly complex mixture using SerialRED.
Detected ultra-low content zeolite phases that are undetectable by X-ray diffraction.
Differentiated phases with identical crystal morphology and similar unit cell parameters.

Conclusions:

SerialRED enables high-throughput phase analysis by rapidly examining hundreds of crystals.
This technique overcomes previous limitations, facilitating the exploration of complex synthesis systems.
SerialRED offers new opportunities for the rapid development of polycrystalline materials.