Screening of anti-fatigue active ingredients of Eleutherococcus senticosus via spectrum-effect relationship based on
Jiabo Cheng1, Lequn Qiu1, Naveed Ahmad2
1Key Laboratory of Forest Plant Ecology, Ministry of Education, Chemical Engineering and Resource Utilization; Engineering Research Center of Forest Bio-preparation, Ministry of Education, Northeast Forestry University; Heilongjiang Provincial Key Laboratory of ecological utilization of Forestry-based active substances, Harbin, China.
Abstract:
ES contains compounds known to have significant anti-fatigue activity. In recent years, it has received extensive attention because it is efficient. However, its active ingredients on antifatigue effect are still unclear. This study attempts to establish the spectrum-effect relationship of ES antifatigue activity to screen the effective components. The results showed that the similarity of 15 ES fingerprints obtained by LC-MS/MS was 0.533-0.992, and the chemical structures of 22 common peaks were identified. The anti-fatigue activity of 15 batches of ES was characterized by forced swimming test of mice and quantified by CAFI, among which S4, S1 and S5 had better activity. 9 components (caffeic acid, 5-(4-O-β-D-glucosylferoyl)-quinic acid, (±)13-HODE, isofraxidin, eleutheroside E, syringin, pinoresinol diglucoside or its isomer, 7,8-dihydrodehydrocarbinol alcohol-4-O-β-D-glucoside, secoisolariciresinol-4-O-β-D-glucoside) highly related to anti-fatigue activity may be the effective components of ES.
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