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Genomic analysis of Enterococcus durans NT21, a putative bacteriocin-producing isolate
Nashwa Tarek1,2, Ahmed O El-Gendy3, Ahmed S Khairalla3,4
1Department of Botany and Microbiology, Faculty of Science, Beni-Suef University, Egypt.
Molecular Biology Research Communications
|January 31, 2023
Summary
Enterococcus durans NT21, a rare bacterial species, was assessed for safety. Whole-genome sequencing revealed no virulence factors, suggesting its potential as a safe probiotic for humans and animals.
Area of Science:
- Microbiology
- Genomics
- Food Safety
Background:
- Enterococcus species are normal commensal bacteria.
- Enterococcus durans is rarely isolated from humans and animals.
- This study focuses on the safety of E. durans NT21.
Purpose of the Study:
- To evaluate the safety of Enterococcus durans NT21.
- To identify potential probiotic properties of E. durans NT21.
Main Methods:
- Whole-genome sequencing (WGS) of E. durans NT21.
- Bioinformatic analysis to identify genes (bacteriocin, virulence, antibiotic resistance, CRISPR-Cas).
- Assessment of physicochemical features and antimicrobial activity.
Main Results:
- E. durans NT21 lacks virulence genes, pathogenicity islands, and plasmids.
- The strain possesses only two antibiotic resistance genes.
- It produces three bacteriocin-like inhibitory substances (Enterolysin A, P, and L50a).
- Six gene-encoded CRISPR-Cas systems and one CRISPR-Cas gene cluster were identified.
Conclusions:
- Enterococcus durans NT21 is a potential probiotic candidate.
- The strain demonstrates safety for both human and animal consumption.
